College of Pharmacy, Chungbuk National University, Cheongju 361-763, Korea.
Arch Pharm Res. 2012 Feb;35(2):367-73. doi: 10.1007/s12272-012-0218-4. Epub 2012 Feb 28.
This experiment was performed to investigate whether apigenin has hypnotic effects and/or enhances pentobarbital-induced sleep behaviors through the GABAergic systems. Apigenin prolonged sleep time induced by pentobarbital similar to muscimol, a GABA(A) receptors agonist. Apigenin also increased sleep rate and sleep time in the combined administration with pentobarbital at the sub-hypnotic dosage, and showed synergic effects with muscimol in potentiating sleep onset and enhancing sleep time induced by pentobarbital. In addition, both of apigeinin and pentobarbital increased chloride influx in primary cultured cerebellar granule cells. Apigenin increased glutamate decarboxylase (GAD) and had no effect on the expression of GABA(A) receptor α-, β-, γ-subunits in n hippocampus of mouse brain, showing different expression of subunits from pentobarbital treatment group. In conclusion, it is suggested that apigenin augments pentobarbital-induced sleep behaviors through chloride ion channel activation.
这项实验旨在研究芹菜素是否通过 GABA 能系统具有催眠作用和/或增强戊巴比妥诱导的睡眠行为。芹菜素延长戊巴比妥诱导的睡眠时间类似于 GABA(A) 受体激动剂 muscimol。芹菜素还增加戊巴比妥在亚催眠剂量下的睡眠率和睡眠时间，并与 muscimol 表现出协同作用，增强戊巴比妥诱导的睡眠起始和增加睡眠时间。此外，芹菜素和戊巴比妥均可增加原代培养小脑颗粒细胞中的氯离子内流。芹菜素增加谷氨酸脱羧酶 (GAD)，对小鼠脑海马 GABA(A) 受体 α-、β-、γ-亚单位的表达没有影响，与戊巴比妥处理组的亚单位表达不同。总之，研究结果表明，芹菜素通过氯离子通道激活增强戊巴比妥诱导的睡眠行为。
