Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Harvard University, Cambridge, Massachusetts, USA.
Nat Biotechnol. 2012 Feb 26;30(3):261-4. doi: 10.1038/nbt.2141.
Insulin-expressing cells that have been differentiated from human pluripotent stem cells in vitro lack the glucose responsiveness characteristic of mature beta cells. Beta-cell maturation in mice was studied to find genetic markers that enable screens for factors that induce bona fide beta cells in vitro. We find that functional beta-cell maturation is marked by an increase in the glucose threshold for insulin secretion and by expression of the gene urocortin 3.
在体外从人类多能干细胞分化而来的表达胰岛素的细胞缺乏成熟β细胞的葡萄糖反应性特征。为了寻找能够在体外诱导真正的β细胞的因子的筛选遗传标记，研究了小鼠β细胞的成熟。我们发现，功能性β细胞成熟的标志是胰岛素分泌的葡萄糖阈值增加和 Urocortin 3 基因的表达。
