成纤维细胞激活蛋白在人胰腺腺癌中的表达及其临床病理意义。
Expression of fibroblast activation protein in human pancreatic adenocarcinoma and its clinicopathological significance.
机构信息
Department of Pathology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
出版信息
World J Gastroenterol. 2012 Feb 28;18(8):840-6. doi: 10.3748/wjg.v18.i8.840.
Abstract
AIM
To examine fibroblast activation protein (FAP) expression in pancreatic ductal adenocarcinoma (PDAC) and to analyze its relationship with the clinicopathology of PDAC.
METHODS
FAP expression was examined in 134 PDAC specimens by immunohistochemistry, and in four pancreatic cancer cell lines (SW1990, Miapaca-2, AsPC-1 and BxPC-3) by Western blotting assay. We also analyzed the association between FAP expression in PDAC cells and the clinicopathology of PDAC patients.
RESULTS
The results showed that the FAP was ex-pressed in both stromal fibroblast cells (98/134, 73.1%) and carcinoma cells (102/134, 76.1%). All 4 pancreatic cancer cell lines expressed FAP protein at different levels. Protein bands corresponding to the proteolytically active 170-kDa seprase dimer and its 88-kDa seprase subunit were identified. Higher FAP expression in carcinoma cells was associated with tumor size (P < 0.001), fibrotic focus (P = 0.003), perineural invasion (P = 0.009) and worse clinical outcome (P = 0.0085).
CONCLUSION
FAP is highly expressed in carcinoma cells and fibroblasts in PDAC tissues, and its expression is associated with desmoplasia and worse prognosis.
摘要
目的
检测胰腺导管腺癌（PDAC）中成纤维细胞激活蛋白（FAP）的表达，并分析其与 PDAC 临床病理的关系。
方法
采用免疫组织化学法检测 134 例 PDAC 标本中 FAP 的表达，采用 Western blot 法检测 4 株胰腺癌细胞系（SW1990、Miapaca-2、AsPC-1 和 BxPC-3）中 FAP 的表达。我们还分析了 PDAC 细胞中 FAP 表达与 PDAC 患者临床病理的关系。
结果
结果显示，FAP 在间质成纤维细胞（98/134，73.1%）和癌细胞（102/134，76.1%）中均有表达。4 株胰腺癌细胞系均不同程度地表达 FAP 蛋白。鉴定到相应的蛋白条带，即具有蛋白水解活性的 170kDa seprase 二聚体及其 88kDa seprase 亚基。癌细胞中 FAP 的高表达与肿瘤大小（P<0.001）、纤维灶（P=0.003）、神经周围浸润（P=0.009）和较差的临床结局（P=0.0085）有关。
结论
FAP 在 PDAC 组织的癌细胞和成纤维细胞中高表达，其表达与促结缔组织增生和预后不良有关。
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