非平衡同位素交换揭示了 AAA(+) ATP 酶镁螯合酶 ATP 水解途径中具有催化意义的酶-磷酸复合物。
Nonequilibrium isotope exchange reveals a catalytically significant enzyme-phosphate complex in the ATP hydrolysis pathway of the AAA(+) ATPase magnesium chelatase.
机构信息
Department of Chemistry, University of Sheffield, Sheffield S3 7HF, UK.
出版信息
Biochemistry. 2012 Mar 13;51(10):2029-31. doi: 10.1021/bi300149z. Epub 2012 Feb 29.
PMID:22372406
Abstract
Magnesium chelatase is an AAA(+) ATPase that catalyzes the first committed step in chlorophyll biosynthesis. Using nonequilibrium isotope exchange, we show that the ATP hydrolysis reaction proceeds via an enzyme-phosphate complex. Exchange from radiolabeled phosphate to ATP was not observed, offering no support for an enzyme-ADP complex.
摘要
镁螯合酶是一种 AAA(+)ATP 酶，催化叶绿素生物合成的第一步。我们利用非平衡同位素交换，表明 ATP 水解反应通过酶-磷酸复合物进行。未观察到放射性标记的磷酸向 ATP 的交换，这并不支持酶-ADP 复合物的存在。
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