Department of Animal Biology and Marine Ecology, University of Messina, Messina, Italy.
J Appl Microbiol. 2012 Jun;112(6):1102-12. doi: 10.1111/j.1365-2672.2012.05272.x. Epub 2012 Apr 10.
To characterize bacilli isolated from shallow hydrothermal vents of Panarea Island (Italy) and evaluate their biotechnological potential.
Fifteen isolates were characterized by culture and molecular methods. Eleven isolates were thermophilic, six isolates were alkalophilic and four of them were haloalkalophilic. After 16S rRNA gene sequencing, four strains, exhibiting sequence similarity below 95% with deposited strains, may represent novel species of bacilli. One strain was strictly related to Geobacillus subterraneus, but shared phenotypic characteristics for which it could be considered a new strain of this species. Four strains were affiliated with different Bacillus spp. Most isolates produced gelatinase, lipases and amylase, and some were mercury tolerant. Exopolysaccharides (EPS) production was tested adding different sugars (glucose, sucrose, trehalose, fructose, ribose, xylose and mannose, 1% w/v) as a carbon source in a minimal medium. The highest EPS yield (185 mg l(-1)) was reached by strain 1A70 utilizing ribose as a carbon source.
Novel strains of Geobacillus and indigenous ribotypes of Bacillus with biotechnological potential inhabit shallow vents of Panarea Island.
New strains of thermophilic bacilli from Panarea are producers of useful biomolecules for industrial purposes as well as environmental and biotechnological applications.
对来自意大利帕拉雷亚岛浅热液喷口的芽孢杆菌进行分离，并评估其生物技术潜力。
通过培养和分子方法对 15 个分离株进行了特征描述。其中 11 个分离株为嗜热菌，6 个分离株为嗜碱菌，其中 4 个为耐盐嗜碱菌。16S rRNA 基因测序后，4 株与已发表菌株的序列相似性低于 95%，可能代表芽孢杆菌的新种。其中一株与地芽孢杆菌亲缘关系密切，但表现出一些可视为该物种新菌株的表型特征。其余 4 株与不同的芽孢杆菌种相关。大多数分离株产生明胶酶、脂肪酶和淀粉酶，部分菌株对汞具有耐受性。在基础培养基中添加不同的糖（葡萄糖、蔗糖、海藻糖、果糖、核糖、木糖和甘露糖，1%w/v）作为碳源，测试了胞外多糖（EPS）的产生情况。利用核糖作为碳源时，菌株 1A70 的 EPS 产量最高（185mg/L）。
帕拉雷亚岛浅热液喷口栖息着具有生物技术潜力的新型地芽孢杆菌和土著型芽孢杆菌。
来自帕拉雷亚岛的新型嗜热芽孢杆菌菌株是工业、环境和生物技术应用中有用生物分子的生产者。
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