Department of Biological and Environmental Sciences, University of Messina, V.le F. Stagno d'Alcontres 31, 98166 Messina, Italy.
Curr Microbiol. 2013 Jul;67(1):21-9. doi: 10.1007/s00284-013-0327-4. Epub 2013 Feb 10.
A haloalkaliphilic, thermophilic Bacillus strain (T14), isolated from a shallow hydrothermal vent of Panarea Island (Italy), produced a new exopolysaccharide (EPS). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain T14 was highly related (99 % similarity) to Bacillus licheniformis DSM 13(T) and Bacillus sonorensis DSM 13779(T). Further DNA-DNA hybridization analysis revealed 79.40 % similarity with B. licheniformis DSM 13(T) and 39.12 % with B. sonorensis DSM 13779(T). Sucrose (5 %) was the most efficient carbon source for growth and EPS production. The highest EPS production (366 mg l(-1)) was yielded in fermenter culture at 300 rpm after 48 h of incubation. The purified fraction EPS1 contained fructose/fucose/glucose/galactosamine/mannose in a relative proportion of 1.0:0.75:0.28:tr:tr and possessed a molecular weight of 1,000 kDa displaying a trisaccharide unit constituted by sugars with a β-manno-pyranosidic configuration. Screening for biological activity showed anti-cytotoxic effect of EPS1 against Avarol in brine shrimp test, indicating a potential use in the development of novel drugs.
一株来自意大利帕拉雷岛浅海热液喷口的嗜碱嗜热芽孢杆菌（T14）产生了一种新的胞外多糖（EPS）。基于 16S rRNA 基因序列的系统发育分析表明，菌株 T14 与地衣芽孢杆菌 DSM 13（T）和索诺拉芽孢杆菌 DSM 13779（T）高度相关（99%相似性）。进一步的 DNA-DNA 杂交分析显示与地衣芽孢杆菌 DSM 13（T）的相似度为 79.40%，与索诺拉芽孢杆菌 DSM 13779（T）的相似度为 39.12%。蔗糖（5%）是生长和 EPS 生产的最有效碳源。在发酵罐培养中，转速为 300rpm，培养 48 小时后，EPS1 的产量最高（366mg l(-1))。纯化的 EPS1 级分含有果糖/木糖/葡萄糖/半乳糖胺/甘露糖，相对比例为 1.0:0.75:0.28:tr:tr，分子量为 1000kDa，具有β-甘露吡喃糖构型的糖组成的三糖单元。生物活性筛选表明，EPS1 对盐水虾试验中的 Avarol 具有抗细胞毒性作用，表明其在新型药物开发中有潜在用途。
