Jin R Z, Breslauer K J, Jones R A, Gaffney B L
Department of Chemistry, Rutgers, State University of New Jersey, Piscataway 08855.
Science. 1990 Oct 26;250(4980):543-6. doi: 10.1126/science.2237404.
A combination of spectroscopic and calorimetric techniques has been used to characterize the structures formed by a family of short, guanine-containing DNA single strands of the form d[GGTTXTTGG], X = A, C, G, T. In 1 molar NaCl at low temperatures, these molecules do not behave like single strands, but rather exhibit properties consistent with tetraplex formation. The standard state enthalpies, entropies, and free energies for formation of each tetraplex have been measured, as have preliminary nuclear magnetic resonance (NMR) spectra. In 1 molar KCl, the melting behavior of the structure or structures is more complex than in 1 molar NaCl. This observation may be related to the recently proposed "sodium-potassium switch."
已使用光谱学和量热法技术的组合来表征由一系列短的、含鸟嘌呤的d[GGTTXTTGG]形式的DNA单链形成的结构，其中X = A、C、G、T。在低温下的1摩尔氯化钠中，这些分子的行为不像单链，而是表现出与四链体形成一致的性质。已测量了每个四链体形成的标准状态焓、熵和自由能，以及初步的核磁共振（NMR）光谱。在1摩尔氯化钾中，一种或多种结构的熔解行为比在1摩尔氯化钠中更复杂。这一观察结果可能与最近提出的“钠钾开关”有关。
