D'Angelo Giovanni, Vicinanza Mariella, Wilson Cathal, De Matteis Maria Antonietta
Telethon Institute of Genetics and Medicine, Via Pietro Castellino, 111, 80131, Napoli, Italy,
Subcell Biochem. 2012;59:255-70. doi: 10.1007/978-94-007-3015-1_8.
The Golgi complex is a ribbon-like organelle composed of stacks of flat cisternae interconnected by tubular junctions. It occupies a central position in the endomembrane system as proteins and lipids that are synthesized in the endoplasmic reticulum (ER) pass through the Golgi complex to undergo biosynthetic modification (mainly glycosylation) and to be sorted to their final destinations. In addition the Golgi complex possesses a number of activities, apparently not directly connected with its main role in trafficking and sorting, which have been recently reviewed in Wilson et al. 2011. In spite of the constant massive flux of material the Golgi complex maintains its identity and phosphoinositides (PIs), among other factors, play a central role in this process. The active metabolism of PIs at the Golgi is necessary for the proper functioning of the organelle both in terms of membrane trafficking/sorting and its manifold metabolic and signalling activities. Phosphatidylinositol 4-phosphate (PtdIns4P), in particular, is responsible for the recruitment of numerous cytosolic proteins that recognise and bind PtdIns4P via specific lipid-binding domains. In this chapter we will summarize the findings that have contributed to our current understanding of the role of PIs in the biology of the Golgi complex in terms of the regulation of PI metabolism and the functional roles and regulation of PtdIns4P effectors.
高尔基体是一种带状细胞器，由扁平的囊泡堆叠而成，通过管状连接相互连通。它在内膜系统中占据中心位置，因为在内质网（ER）中合成的蛋白质和脂质会穿过高尔基体进行生物合成修饰（主要是糖基化）并被分选到它们的最终目的地。此外，高尔基体还具有许多活动，这些活动显然与其在运输和分选方面的主要作用没有直接联系，Wilson等人在2011年对此进行了综述。尽管有持续大量的物质流动，高尔基体仍保持其特性，磷酸肌醇（PIs）等因素在这一过程中起着核心作用。高尔基体中PI的活跃代谢对于该细胞器在膜运输/分选及其多种代谢和信号传导活动方面的正常功能是必要的。特别是磷脂酰肌醇4-磷酸（PtdIns4P），它负责募集众多通过特定脂质结合域识别并结合PtdIns4P的胞质蛋白。在本章中，我们将总结一些研究结果，这些结果有助于我们目前对PI在高尔基体生物学中的作用的理解，包括PI代谢的调节以及PtdIns4P效应器的功能作用和调节。
