Furukawa Y, Piwnica-Worms H, Ernst T J, Kanakura Y, Griffin J D
Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, MA 02115.
Science. 1990 Nov 9;250(4982):805-8. doi: 10.1126/science.2237430.
The product of the cdc2 gene, designated p34cdc2, is a serine-threonine protein kinase that controls entry of eukaryotic cells into mitosis. Freshly isolated human T lymphocytes (G0 phase) were found to have very low amounts of p34cdc2 and cdc2 messenger RNA. Expression of cdc2 increased 18 to 24 hours after exposure of T cells to phytohemagglutinin, coincident with the G1 to S transition. Antisense oligodeoxynucleotides could reduce the increase in cdc2 expression and inhibited DNA synthesis, but had no effect on several early and mid-G1 events, including blastogenesis and expression of interleukin-2 receptors, transferrin receptors, c-myb, and c-myc. Induction of cdc2 required prior induction of c-myb and c-myc. These results suggest that cdc2 induction is part of an orderly sequence of events that occurs at the G1 to S transition in T cells.
细胞分裂周期蛋白2（cdc2）基因的产物，即p34cdc2，是一种丝氨酸 - 苏氨酸蛋白激酶，它控制真核细胞进入有丝分裂。新鲜分离的人T淋巴细胞（G0期）被发现含有极少量的p34cdc2和cdc2信使核糖核酸。T细胞暴露于植物血凝素后18至24小时，cdc2的表达增加，这与从G1期到S期的转变同时发生。反义寡脱氧核苷酸可以减少cdc2表达的增加并抑制DNA合成，但对几个G1期早期和中期事件没有影响，包括细胞增殖和白细胞介素 - 2受体、转铁蛋白受体、c - myb和c - myc的表达。cdc2的诱导需要先诱导c - myb和c - myc。这些结果表明cdc2的诱导是T细胞从G1期到S期转变时发生的一系列有序事件的一部分。
