Ueda A, Nagasawa K, Tsukamoto H, Kushimoto K, Ishii Y, Yamauchi Y, Tada Y, Niho Y, Kusaba T, Volanakis J E
First Department of Internal Medicine, Kyushu University, Fukuoka-City.
Ryumachi. 1990 Apr;30(2):103-8.
A sensitive solid-phase radioimmunoassay for intact complement protein B and its smaller fragment Ba is described which quantitates human B and Ba to 30 ng/ml. The assay was proved to be useful to evaluate the activation of the alternative pathway of the complement system by measuring intact B and Ba in normal human sera activated with zymosan. In sera from patients with systemic lupus erythematosus (SLE), the level of intact B was decreased and that of Ba was increased in the active phase, and the level of Ba was increased though intact B which was within normal limits in the inactive phase. The present result suggests that the activation of the alternative pathway is occurring both in the active and inactive phase in SLE, and the measurement of intact B and Ba concentration in pathological sera would be useful to evaluate the activation of the alternative complement pathway.
本文描述了一种用于完整补体蛋白B及其较小片段Ba的灵敏固相放射免疫测定法，该方法可将人B和Ba定量至30 ng/ml。通过测量用酵母聚糖激活的正常人血清中的完整B和Ba，证明该测定法可用于评估补体系统替代途径的激活。在系统性红斑狼疮（SLE）患者的血清中，活动期完整B水平降低，Ba水平升高，而在非活动期，完整B水平在正常范围内，但Ba水平升高。目前的结果表明，SLE的活动期和非活动期均发生替代途径的激活，测量病理血清中完整B和Ba的浓度将有助于评估替代补体途径的激活。
