Department of Oral and Maxillofacial Surgery, Facultad de Medicina, Universidad de Oviedo, Hospital Universitario Central de Asturias,c/Catedrático José Serrano s/n, Oviedo, Asturias, Spain.
Oral Oncol. 2012 Jul;48(7):641-6. doi: 10.1016/j.oraloncology.2012.02.004. Epub 2012 Feb 28.
There is a need for novel and accurate biomarkers based on genetic abnormalities capable of predicting the risk of malignant transformation of epithelial lesions of the oral cavity. Therefore, we investigate the role of cortactin and focal adhesion kinase (FAK) protein expression in oral dysplasias and their potential utility as cancer risk markers. Cortactin and FAK expression were immunohistochemically evaluated in 64 patients with oral epithelial dysplasia. During follow-up, 17 of 64 patients developed an oral squamous cell carcinoma (OSCC). Increased immunoexpression of cortactin and FAK was found in 52 and 27 of 64 oral dysplasias, respectively, and the expression of both proteins increased with the grade of dysplasia. Increased cortactin and FAK expression was also observed in 13 and 15 OSCC, respectively. Overall, cortactin and FAK expression was maintained or further augmented in the oral squamous cell carcinoma compared to the patient-matched preinvasive lesion. Univariate analysis showed that cortactin and FAK expression, as well as histological grading were significantly associated with oral cancer risk. Strong coexpression of both proteins reflected a significantly higher cancer risk than that of weak to moderate expression or than whenever only one of those proteins showed a strong expression. In multivariate analysis, premalignant oral lesions which exhibited a high coexpression of cortactin and FAK showed a significant risk of developing an OSCC (HR=6.298). Our results indicate that strong immunoexpression of cortactin and FAK, and not only one of them, is a predicting factor for increased cancer risk in oral premalignant lesions.
需要基于遗传异常的新型和准确生物标志物来预测口腔上皮病变恶性转化的风险。因此，我们研究了桩蛋白和粘着斑激酶（FAK）蛋白表达在口腔发育不良中的作用及其作为癌症风险标志物的潜在用途。我们用免疫组织化学方法评估了 64 例口腔上皮发育不良患者的桩蛋白和 FAK 表达。在随访中，64 例患者中有 17 例发展为口腔鳞状细胞癌（OSCC）。在 64 例口腔发育不良中，分别有 52 例和 27 例发现桩蛋白和 FAK 的免疫表达增加，并且这两种蛋白的表达随着发育不良的程度而增加。在 13 例和 15 例 OSCC 中也观察到桩蛋白和 FAK 的表达增加。总体而言，与患者匹配的癌前病变相比，桩蛋白和 FAK 在口腔鳞状细胞癌中的表达保持或进一步增强。单因素分析显示，桩蛋白和 FAK 表达以及组织学分级与口腔癌风险显著相关。两种蛋白的强共表达比弱至中度表达或仅其中一种蛋白强表达时的癌症风险更高。在多变量分析中，显示桩蛋白和 FAK 高共表达的癌前口腔病变发生 OSCC 的风险显著增加（HR=6.298）。我们的结果表明，桩蛋白和 FAK 的强免疫表达，而不仅仅是其中之一，是口腔癌前病变癌症风险增加的预测因素。
