蜜环菌提取物抑制单纯疱疹病毒体外附着。

Melissa officinalis extract inhibits attachment of herpes simplex virus in vitro.

机构信息

Department of Infectious Diseases, Virology, University of Heidelberg, Heidelberg, Germany.

出版信息

Chemotherapy. 2012;58(1):70-7. doi: 10.1159/000335590. Epub 2012 Feb 23.

DOI:10.1159/000335590

PMID:22377592

Abstract

BACKGROUND

Extracts and essential oils of medicinal plants are increasingly of interest as novel drugs for antiherpetic agents, since the herpes simplex virus (HSV) might develop resistance to commonly used antiviral drugs.

METHODS

An aqueous extract of Melissa officinalis as well as phenolic extract compounds, i.e. caffeic acid, p-coumaric acid and rosmarinic acid were examined for their antiviral activity against herpes simplex virus type 1 (HSV-1) in vitro.

RESULTS

When drugs were added to HSV-1-infected cells, no antiviral effect was observed as determined by plaque reduction assay and analysis of expression of viral protein ICP0. However, the Melissa extract demonstrated a high virucidal activity against HSV-1, even at very low concentrations of 1.5 μg/ml, whereas similar results for phenolic compounds were only achieved at 100 times higher concentrations. Besides the virucidal activity, the Melissa extract and rosmarinic acid inhibited HSV-1 attachment to host cells in a dose-dependent manner. These results indicate that rosmarinic acid was the main contributor to the antiviral activity of Melissa extract. However, the selectivity index of Melissa extract of 875 against HSV is superior to the selectivity indices of single constituents.

CONCLUSION

Melissa extract exhibits low toxicity, is virucidal and affects HSV-1 attachment to host cells in vitro.

摘要

背景

由于单纯疱疹病毒（HSV）可能对常用的抗病毒药物产生耐药性，因此药用植物的提取物和精油越来越受到作为新型抗疱疹药物的关注。

方法

本研究检测了药用薰衣草水提物以及酚酸提取物化合物，如咖啡酸、对香豆酸和迷迭香酸，对体外单纯疱疹病毒 1 型（HSV-1）的抗病毒活性。

结果

通过噬斑减少试验和病毒蛋白 ICP0 表达分析，当药物被添加到 HSV-1 感染的细胞中时，没有观察到抗病毒作用。然而，即使在非常低的 1.5μg/ml 浓度下，薰衣草提取物也表现出对 HSV-1 的高病毒杀伤活性，而类似的酚类化合物结果仅在 100 倍更高的浓度下实现。除了病毒杀伤活性外，薰衣草提取物和迷迭香酸还以剂量依赖的方式抑制 HSV-1 对宿主细胞的附着。这些结果表明迷迭香酸是薰衣草提取物抗病毒活性的主要贡献者。然而，薰衣草提取物对 HSV 的选择性指数 875 优于单个成分的选择性指数。