Department of Chemistry, Kongju National University, Gongju 314-701, Republic of Korea.
Bioorg Med Chem. 2012 Apr 1;20(7):2376-81. doi: 10.1016/j.bmc.2012.02.002. Epub 2012 Feb 9.
Four new quercetin acylglycosides, designated camelliquercetisides A-D, quercetin 3-O-[α-L-arabinopyranosyl(1→3)][2-O″-(E)-p-coumaroyl][β-D-glucopyranosyl(1→3)-α-L-rhamnopyranosyl(1→6)]-β-D-glucoside (17), quercetin 3-O-[2-O″-(E)-p-coumaroyl][β-D-glucopyranosyl(1→3)-α-L-rhamnopyranosyl(1→6)]-β-D-glucoside (18), quercetin 3-O-[α-L-arabinopyranosyl(1→3)][2-O″-(E)-p-coumaroyl][α-L-rhamnopyranosyl(1→6)]-β-d-glucoside (19), and quercetin 3-O-[2-O″-(E)-p-coumaroyl][α-L-rhamnopyranosyl(1→6)]-β-D-glucoside (20), together with caffeine and known catechins, and flavonoids (1-16) were isolated from the leaves of Camellia sinensis. Their structures were determined by spectroscopic (1D and 2D NMR, IR, and HR-TOF-MS) and chemical methods. The catechins and flavonoidal glycosides exhibited yeast alcohol dehydrogenase (ADH) inhibitory activities in the range of IC(50) 8.0-70.3μM, and radical scavenging activities in the range of IC(50) 1.5-43.8 μM, measured by using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical.
从茶树的叶子中分离得到四个新的槲皮素酰基糖苷，分别命名为茶陵槲皮苷 A-D，槲皮素 3-O-[α-L-阿拉伯吡喃糖基(1→3)][2-O″-(E)-对香豆酰基][β-D-吡喃葡萄糖基(1→3)-α-L-鼠李吡喃糖基(1→6)]-β-D-吡喃葡萄糖苷（17），槲皮素 3-O-[2-O″-(E)-对香豆酰基][β-D-吡喃葡萄糖基(1→3)-α-L-鼠李吡喃糖基(1→6)]-β-D-吡喃葡萄糖苷（18），槲皮素 3-O-[α-L-阿拉伯吡喃糖基(1→3)][2-O″-(E)-对香豆酰基][α-L-鼠李吡喃糖基(1→6)]-β-D-吡喃葡萄糖苷（19）和槲皮素 3-O-[2-O″-(E)-对香豆酰基][α-L-鼠李吡喃糖基(1→6)]-β-D-吡喃葡萄糖苷（20），以及咖啡因和已知的儿茶素和类黄酮（1-16）。它们的结构通过光谱（1D 和 2D NMR、IR 和 HR-TOF-MS）和化学方法确定。儿茶素和类黄酮糖苷在酵母醇脱氢酶（ADH）抑制活性方面表现出范围为 IC50 8.0-70.3μM 的活性，在自由基清除活性方面表现出范围为 IC50 1.5-43.8 μM 的活性，通过使用 1,1-二苯基-2-苦基肼（DPPH）自由基测量。
