Rodeghiero F, Castaman G C, Tosetto A, Lattuada A, Mannucci P M
Department of Hematology, San Bortolo Hospital, Vicenza, Italy.
Thromb Res. 1990 Jul 15;59(2):259-67. doi: 10.1016/0049-3848(90)90129-z.
Two different methods (using Triton X-100 and glycerol) for lysing platelets to measure platelet vWF concentrations were compared directly. The platelet concentration of von Willebrand factor antigen (vWF:Ag) was similar for both methods, whereas ristocetin cofactor activity (Ricof) was higher with Triton than with glycerol. After storing platelet lysates for two months at -80 degrees C vWF:Ag and Ricof concentrations decreased with both methods of lysis. Larger than normal (supranormal) vWF multimeric forms could be visualized in platelet lysates obtained using both methods, with no change of the multimeric pattern during storage. Triton can be recommended as the agent of choice to lyse platelets for measurement of their vWF concentration, but the samples must be assayed within two weeks to avoid decay of Ricof activity.
直接比较了两种不同的裂解血小板以测量血小板血管性血友病因子（vWF）浓度的方法（使用曲拉通X-100和甘油）。两种方法测得的血管性血友病因子抗原（vWF:Ag）的血小板浓度相似，而用曲拉通法测得的瑞斯托霉素辅因子活性（Ricof）高于甘油法。在-80℃下将血小板裂解物储存两个月后，两种裂解方法测得的vWF:Ag和Ricof浓度均下降。使用两种方法获得的血小板裂解物中均可观察到大于正常的（超正常的）vWF多聚体形式，且储存期间多聚体模式无变化。推荐使用曲拉通作为裂解血小板以测量其vWF浓度的首选试剂，但样品必须在两周内进行检测，以避免Ricof活性下降。
