Changes in morphology of long bone marrow tissue of rats submitted to cryotherapy with liquid nitrogen.
作者信息
Costa Fábio Wildson Gurgel, Pessoa Rosana Maria Andrade, Nogueira Carlos Bruno Pinheiro, Pereira Karuza Maria Alves, Brito Gerly Anne de Castro, Soares Eduardo Costa Studart
机构信息
Division of Stomatology, UFC Sobral Campus, Fortaleza, CE, Brazil.
出版信息
Acta Cir Bras. 2012 Feb;27(2):179-84. doi: 10.1590/s0102-86502012000200014.
PURPOSE
To study the main effects of local use of liquid nitrogen on bone marrow tissue in rats.
METHODS
The femoral diaphyses of 42 Wistar rats were exposed to three local and sequential applications of liquid nitrogen for one or two minutes, intercalated with periods of five minutes of passive thawing. The animals were sacrificed after one, two, four and 12 weeks and the specimens obtained were analyzed histomorphologically.
RESULTS
In the second experimental week of one-minute protocol, histological degree of inflammation obtained a mean score of one (mild), ranging from 0 (absent or scarce) and two (moderate) (Kruskal-Wallis test p=0.01). In the second experimental week of two-minute protocol, degree of inflammation to the medullar tissue obtained an average score of two (Kruskal-Wallis test p=0.01).
CONCLUSION
The degree of inflammation of the bone marrow tissue was higher in protocol of three applications of two minutes compared to protocol of three applications of one minute.
Zotero 插件