La Rosa Giuseppina, Fratini Marta, Spuri Vennarucci Valentina, Guercio Annalisa, Purpari Giuseppa, Muscillo Michele
Department of Environment and Primary Prevention, Istituto Superiore di Sanita, Rome, Italy.
New Microbiol. 2012 Jan;35(1):27-34. Epub 2012 Jan 10.
We evaluated the presence of the enteric viruses: norovirus, adenovirus, enterovirus, astrovirus, hepatitis A virus, and hepatitis E virus in bivalves using nested PCR methods and cell culture assays. Noroviruses GII.4 and GIV.1, adenoviruses types 1 and 2, hepatitis A, and echovirus type 7 were detected in the shellfish tested, which were often co-infected. This is the first study to detect such a high level of viral contamination in Italian mussels (up to four different viral groups in a single sample), and the first to document the presence of GIV NoV in shellfish.
我们使用巢式PCR方法和细胞培养试验评估了双壳贝类中肠道病毒的存在情况，这些病毒包括诺如病毒、腺病毒、肠道病毒、星状病毒、甲型肝炎病毒和戊型肝炎病毒。在所检测的贝类中发现了诺如病毒GII.4和GIV.1、1型和2型腺病毒、甲型肝炎病毒以及7型艾柯病毒，这些贝类常常受到多种病毒的共同感染。这是首次在意大利贻贝中检测到如此高水平的病毒污染（单个样本中多达四种不同病毒组），也是首次记录到贝类中存在GIV NoV。
