Wu Fie
Shanghai Changzheng Hospital, Shanghai, 200003.
Zhongguo Yi Liao Qi Xie Za Zhi. 2011 Nov;35(6):462-4.
To compare the sensitivity, the specificity and the anti-jamming of several excrement occult blood experimental techniques. To evaluate the effect of transferrin (Tf) in the excrement in the digestive tract hemorrhage detection rate.
For 600 patients of clinical suspicious digestive tract hemorrhage, take their excrement specimen, using the chemical process (pyramidon semi-quantitative examination law) to detect hemoglobin (Hb), and using monoclonal antibody colloidal gold method to detect Hb and Tf.
Finally the hemoglobin chemical process (hereafter refers to as chemical process) to detect upper gastrointestinal hemorrhage with the positive rate 57.3%, and the detection of hemorrhage of lower digestive tract's positive rate is 44.8%; Hemoglobin monoclonal antibody colloidal gold method (hereafter refers to as colloid gold law) to examine upper gastrointestinal hemorrhage with a positive rate 60.4%, under examination hemorrhage with positive rate 77.6%; transferrin monoclonal antibody colloidal gold method (hereafter refer to as transferrin law) to examine upper gastrointestinal hemorrhage with a positive rate 82.3%, examination hemorrhage of lower digestive tract with a positive rate 66.4%; The union examination law (hemoglobin and transferrin to be detected twice, once positive that is positive) examines upper gastrointestinal hemorrhage the positive rate is 90.8%, hemorrhage of lower digestive tract's positive rate is 97.6%.
Excrement transferrin has the high detection rate in the upper gastrointestinal hemorrhage; Hb and the Tf combined examination may obviously raise the digestive tract hemorrhagic disease's positive detection rate.
比较几种粪便潜血实验技术的灵敏度、特异性及抗干扰能力。评价粪便中转铁蛋白(Tf)在消化道出血检测率中的作用。
对600例临床怀疑消化道出血的患者采集粪便标本,采用化学法(匹拉米洞半定量检测法)检测血红蛋白(Hb),采用单克隆抗体胶体金法检测Hb和Tf。
最终血红蛋白化学法(以下简称化学法)检测上消化道出血的阳性率为57.3%,检测下消化道出血的阳性率为44.8%;血红蛋白单克隆抗体胶体金法(以下简称胶体金法)检测上消化道出血的阳性率为60.4%,检测下消化道出血的阳性率为77.6%;转铁蛋白单克隆抗体胶体金法(以下简称转铁蛋白法)检测上消化道出血的阳性率为82.3%,检测下消化道出血的阳性率为66.4%;联合检测法(血红蛋白和转铁蛋白各检测两次,一次阳性即为阳性)检测上消化道出血的阳性率为90.8%,检测下消化道出血的阳性率为97.6%。
粪便转铁蛋白在上消化道出血中有较高的检测率;Hb与Tf联合检测可明显提高消化道出血性疾病的阳性检出率。