He Wei, Li Yan-Hua, Rong Pei-Jing, Li Liang, Ben Hui, Zhu Bing
Department of Psychology, Institute of Acu-moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Zhen Ci Yan Jiu. 2011 Dec;36(6):414-8.
To observe the anti-seizure effect of electroacupuncture (EA) at different sites regions of the auricle in awake rats.
Sixty male SD rats were randomly divided into model, ear-apex, ear-outer-margin, earlobe, cymba-concha, cavitas-conchae groups (n = 10 in each group). A metal microelectrode (diameter: 50 microm) was implanted into the somatosensary cortex for recording the field potentials (FPs). Epileptic seizure model was established by intraperitoneal injection of pentylenetetrazol (PTZ, 60 mg/kg). EA (20 Hz, 1 mA) was applied to ear-apex, ear-outer-margin, earlobe, cymaba-concha and cavitas-conchae for 30 min, respectively. The epileptic behavior changes (scores) were determined in the light of Racine's method, and the epileptic seizure was assessed according to the epileptic FPs (5-folds above the basic amplitude) of the cerebral cortex.
In comparison with the model group, the latency of the first seizure of epilepsy in the ear-outer-margin, earlobe, cymba-concha, cavitas-conchae groups were increased significantly (P < 0.01), while the scores of epileptic behavior and the duration of seizure shown by FPs decreased considerably in the ear-apex, ear-outer-margin, earlobe, cymba-concha, cavitas-conchae groups (P < 0.01). Comparison among the five EA treatment groups showed that the effects of both cymba-concha and cavitas-conchae groups were significantly superior to those of ear-apex, earlobe and ear-outer-margin groups in increasing the latency of the first seizure of epilepsy, and reducing the scores of the epileptic behavior and seizure duration (P < 0.01). No significant differences were found between the cymba-concha and cavitas-conchae groups in the above-mentioned 3 indexes (P > 0.05).
EA of cymba-concha and cavitas-conchae has a good effect in suppressing epileptic seizures, which may be mediated via the auricular branch of the vagus nerve.
观察电针刺激清醒大鼠耳廓不同部位区域的抗癫痫作用。
将60只雄性SD大鼠随机分为模型组、耳尖组、耳外缘组、耳垂组、耳甲艇组、耳甲腔组（每组10只）。将一根金属微电极（直径：50微米）植入体感皮层以记录场电位（FPs）。通过腹腔注射戊四氮（PTZ，60毫克/千克）建立癫痫发作模型。分别将电针（20赫兹，1毫安）施加于耳尖、耳外缘、耳垂、耳甲艇和耳甲腔30分钟。根据Racine方法确定癫痫行为变化（评分），并根据大脑皮层的癫痫场电位（高于基础振幅5倍）评估癫痫发作情况。
与模型组相比，耳外缘组、耳垂组、耳甲艇组、耳甲腔组癫痫首次发作潜伏期显著延长（P < 0.01），而耳尖组、耳外缘组、耳垂组、耳甲艇组、耳甲腔组癫痫行为评分及场电位显示的发作持续时间显著缩短（P < 0.01）。五个电针治疗组之间比较显示，耳甲艇组和耳甲腔组在延长癫痫首次发作潜伏期、降低癫痫行为评分及发作持续时间方面的效果显著优于耳尖组、耳垂组和耳外缘组（P < 0.01）。耳甲艇组和耳甲腔组在上述3项指标上无显著差异（P > 0.05）。
耳甲艇和耳甲腔电针在抑制癫痫发作方面具有良好效果，其机制可能通过迷走神经耳支介导。
