Li Miao, Feng Haiyan, Yang Zhongfang, Liu Changzheng, Xia Xueqi, Wang Chengyu, Jiang Lizhen, Jiang Hongchen
School of the Earth Sciences and Resources, China University of Geosciences, Beijing 100083, China.
Wei Sheng Wu Xue Bao. 2011 Dec;51(12):1595-604.
The diversity of culturable bacteria in the six frozen soils from High-latitude area and High-altitude area were analyzed by using culture-dependent approaches.
Three solidified media were used to isolate culturable bacteria. Bacterial 16S rRNA genes of the isolates were PCR amplified using bacteria-universal primers, and then were sequenced. The resulting bacterial 16S rRNA gene sequences were subjected to phylogenetic analysis.
The abundance of culturable bacteria ranged from 4.70 x 10(3) to 2.57 x 10(5) colony forming units (CFU) per gram of soils (dry weight). A total of 144 bacterial strains were obtained. The bacterial isolates from the High-latitude area were affiliated with three phyla (Firmicutes, Gammaproteobacteria, and Betaproteobacteria) with Pseudomonas, Bacillus, and Paenibacillus strains being dominant. The bacterial isolates from High-altitude area could be grouped into three different phyla (Gammaproteobacteria, Firmicutes, and Bacteroidetes) with Pseudomonas strains be dominant.
The culturable bacteria are abundant and diverse in the frozen soils of High-latitude and High-altitude areas. The results also showed that the culturable bacterial community structure varied among different research areas. Our data have implications for a better understanding of culturable bacterial community in the frozen soils in China.
采用依赖培养的方法分析高纬度地区和高海拔地区6种冻土中可培养细菌的多样性。
使用三种固化培养基分离可培养细菌。使用细菌通用引物对分离株的细菌16S rRNA基因进行PCR扩增，然后进行测序。对所得的细菌16S rRNA基因序列进行系统发育分析。
每克土壤（干重）中可培养细菌的丰度范围为4.70×10³至2.57×10⁵菌落形成单位（CFU）。共获得144株细菌菌株。高纬度地区的细菌分离株隶属于三个门（厚壁菌门、γ-变形菌门和β-变形菌门），以假单胞菌属、芽孢杆菌属和类芽孢杆菌属菌株为主。高海拔地区的细菌分离株可分为三个不同的门（γ-变形菌门、厚壁菌门和拟杆菌门），以假单胞菌属菌株为主。
高纬度和高海拔地区的冻土中可培养细菌丰富多样。结果还表明，不同研究区域的可培养细菌群落结构存在差异。我们的数据有助于更好地了解中国冻土中的可培养细菌群落。
