Nagia Mohamed Ms, El-Metwally Mohammad Magdy, Shaaban Mohamed, El-Zalabani Soheir M, Hanna Atef G
Division of Pharmaceutical Industries, Chemistry of Natural Compounds Department, National Research Centre, El-Behoos st, 33, Dokki, Cairo 12622, Egypt.
Org Med Chem Lett. 2012 Mar 1;2(1):9. doi: 10.1186/2191-2858-2-9.
The chemical constituents and biological activities of the terrestrial Aspergillus flavipes MM2 isolated from Egyptian rice hulls are reported. Seven bioactive compounds were obtained, of which one sterol: ergosterol (1), four butyrolactones: butyrolactone I (2), aspulvinone H (3), butyrolactone-V (6) and 4,4'-diydroxypulvinone (7), along with 6-methylsalicylic acid (4) and the cyclopentenone analogue; terrien (5). Structures of the isolated compounds were deduced by intensive studies of their 1D & 2D NMR, MS data and comparison with related structures. The strain extract and the isolated compounds (1-7) were biologically studied against number of microbial strains, and brine shrimp for cytotoxicity. In this article, the taxonomical characterization of A. flavipes MM2 along with its upscale fermentation, isolation and structural assignment of the obtained bioactive metabolites, and evaluate their antimicrobial and cytotoxic activities were described.
报道了从埃及稻壳中分离出的陆生黄柄曲霉MM2的化学成分和生物活性。获得了七种生物活性化合物，其中一种甾醇：麦角甾醇（1），四种丁内酯：丁内酯I（2）、曲霉酮H（3）、丁内酯-V（6）和4,4'-二羟基曲霉酮（7），以及6-甲基水杨酸（4）和环戊烯酮类似物；土曲霉素（5）。通过对其1D和2D NMR、MS数据的深入研究以及与相关结构的比较，推断出分离化合物的结构。对该菌株提取物和分离出的化合物（1-7）针对多种微生物菌株进行了生物学研究，并对卤虫进行了细胞毒性研究。本文描述了黄柄曲霉MM2的分类学特征及其放大发酵、所获得生物活性代谢产物的分离和结构鉴定，并评估了它们的抗菌和细胞毒性活性。
