Organic and Bioorganic Chemistry, Department of Chemistry, University of Bielefeld, University Str. 25, 33615 Bielefeld, Germany.
Botany and Microbiology Department, Faculty of Science, Damanhour University, Damanhour, Egypt.
Z Naturforsch C J Biosci. 2019 Nov 26;74(11-12):283-288. doi: 10.1515/znc-2019-0010.
In the search for bioactive secondary metabolites from terrestrial fungi, four compounds, namely, 3-methyl-3H-quinazolin-4-one (1), aurantiomide C (2), 3-O-methylviridicatin (3), and dehydrocyclopeptine (4), were isolated from Penicillium sp. 8PKH, fungal strain, isolated from deteriorated rice straws. The structures of the isolated compounds were identified by extensive NMR and mass analyses and comparison with literature data. This is the first report of the structure of 3-methyl-3H-quinazolin-4-one (1) with full NMR spectral data having been previously identified by GC-MS from Piper beetle. Analysis of the non-polar fractions of the strain extract by GC-MS revealed the presence of additional eight compounds: methyl-hexadecanoate, methyl linoleate, methyl-9 (Z)-octadecenoate, methyl-octadecanoate, cis-9-oxabicyclo (6.1.0) nonane, 9,12-octadecadienal (9E,12E), ethyl-(E)-9-octadecenoate, and 3-buten-2-ol. The isolated compounds were evaluated for their antimicrobial and cytotoxic activities and exhibited little or no inhibitory activities against the test strains. The taxonomical characterisation and fermentation of the fungal strain were reported as well.
在寻找陆地真菌中的生物活性次生代谢产物时，从真菌菌株 Penicillium sp. 8PKH 中分离得到了四种化合物，分别为 3-甲基-3H-喹唑啉-4-酮（1）、aurantiamide C（2）、3-O-甲基维替定（3）和脱氢环肽（4）。通过广泛的 NMR 和质谱分析以及与文献数据的比较，确定了分离化合物的结构。这是首次报道 3-甲基-3H-喹唑啉-4-酮（1）的结构，其全 NMR 谱数据先前通过 GC-MS 从 Piper 甲虫中鉴定。通过 GC-MS 对菌株提取物的非极性馏分进行分析，发现了另外八种化合物：己酸甲酯、亚油酸甲酯、9(Z)-十八碳烯酸甲酯、十八酸甲酯、顺式-9-氧杂双环（6.1.0）壬烷、9,12-十八碳二烯醛（9E,12E）、（E）-9-十八碳烯酸乙酯和 3-丁烯-2-醇。对分离得到的化合物进行了抗菌和细胞毒性活性评价，结果表明它们对测试菌株几乎没有抑制活性。同时还报道了真菌菌株的分类特征和发酵情况。
