Hewitt C W, Ramsamooj R, Patel M P, Yazdi B, Achauer B M, Black K S
Department of Surgery, University of California, Irvine 92717.
Transplantation. 1990 Nov;50(5):766-72. doi: 10.1097/00007890-199011000-00006.
A consistent majority (62.5%) of immunologically unmodified rat recipients transplanted with vascularized hind-limb bone marrow allografts across a semiallogeneic transplant barrier developed tolerance with absence of graft-versus-host disease. A minority of recipients (37.5%) demonstrated lethal GVHD. Transplantation tolerance in the majority was associated with the induction of stable low-level mixed T cell chimerism, including donor CD5+, CD4+, and CD8+ lymphocytes. Chimeras were specifically immune nonresponsive to host alloantigenic determinants. These results emphasized a potentially important mechanism for low-level stable mixed lymphoid chimerism (SMLC) in tolerance induction, independent of immune suppressive effects due to irradiation or immunopharmacologic intervention. These vascularized bone marrow transplantation (VBMT) results may establish the experimental foundation for a novel approach to stem cell transfer and bone marrow transplantation.
在跨越半同种异体移植屏障移植血管化后肢骨髓同种异体移植物的免疫未修饰大鼠受体中，持续多数（62.5%）的受体产生了耐受性，且无移植物抗宿主病。少数受体（37.5%）表现出致死性移植物抗宿主病。多数受体的移植耐受性与稳定的低水平混合T细胞嵌合体的诱导有关，包括供体CD5 +、CD4 +和CD8 +淋巴细胞。嵌合体对宿主同种异体抗原决定簇具有特异性免疫无反应性。这些结果强调了低水平稳定混合淋巴细胞嵌合体（SMLC）在耐受性诱导中的一个潜在重要机制，该机制独立于辐射或免疫药理干预产生的免疫抑制作用。这些血管化骨髓移植（VBMT）结果可能为干细胞移植和骨髓移植的新方法奠定实验基础。
