Rudolf-Boehm-Institute of Pharmacology and Toxicology, University of Leipzig, Leipzig, Germany.
J Neurochem. 2012 May;121(4):587-96. doi: 10.1111/j.1471-4159.2012.07718.x. Epub 2012 Mar 21.
Changes in the density of NMDA (GluN) receptors in the neuronal membrane are critical for plasticity, whereas malfunction of precisely regulated GluN receptor activity may be involved in neurotoxicity. In cultured rat neocortical interneurons, we have studied the regulation of the surface density of GluN1, GluN2A and GluN2B subunits. Application of 5 μMol NMDA for 24 h followed by a washout period of 24 h decreased the response of GluN receptors for at least 2 days. The reduction was caused by a decrease in the surface density of GluN1/GluN2B subunits, whereas GluN2A subunits remained unaffected. Our data indicate that long but reversible low level activation of GluN receptors can cause long-term changes in their subunit composition in cultured interneurons.
神经元膜上 NMDA（GluN）受体密度的变化对可塑性至关重要，而精确调节的 GluN 受体活性的功能障碍可能与神经毒性有关。在培养的大鼠新皮层中间神经元中，我们研究了 GluN1、GluN2A 和 GluN2B 亚基表面密度的调节。应用 5μMol NMDA24 小时，随后洗脱 24 小时，至少 2 天内降低了 GluN 受体的反应。这种减少是由 GluN1/GluN2B 亚基表面密度的降低引起的，而 GluN2A 亚基不受影响。我们的数据表明，GluN 受体的长期但可逆的低水平激活可导致培养的中间神经元中其亚基组成发生长期变化。
