Nutrition and Food Science Department, XaRTA, INSA, Pharmacy School, University of Barcelona, 08028 Barcelona, Spain.
J Agric Food Chem. 2012 Apr 4;60(13):3373-80. doi: 10.1021/jf204702f. Epub 2012 Mar 26.
The present study aims to compare the phenolic profiles of organic and conventional tomatoes bought in the market. For the quantification and identification of individual polyphenols, liquid chromatography coupled to mass spectrometry in tandem mode (LC-MS/MS) was carried out. Confirmation of the compounds previously identified on the triple-quadrupole was accomplished by injection in the high-resolution system (QToF-MS). In this way, 34 compounds were identified in tomato fruits. Recoveries of targeted polyphenols exceed 78% for conventional and organic tomatoes, respectively. The method intraday precision ranged between 3 and 5%, whereas the interday one was below 12%. Comparing the analyses of tomatoes from conventional and organic production systems demonstrated statistically higher levels (P < 0.05) of phenolic compounds in organic tomatoes. This methodology allowed finding differences in the bioactive components of organic and conventional tomatoes not previously reported.
本研究旨在比较市场上购买的有机和传统番茄的酚类成分。为了定量和鉴定各个多酚，采用液相色谱-串联质谱法（LC-MS/MS）进行分析。通过在高分辨率系统（QToF-MS）中注入来验证三重四极杆上预先鉴定的化合物。通过这种方式，在番茄果实中鉴定出 34 种化合物。传统和有机番茄中目标多酚的回收率分别超过 78%。该方法日内精密度在 3%至 5%之间，而日间精密度则低于 12%。比较传统和有机生产系统的番茄分析表明，有机番茄中酚类化合物的含量显著更高（P < 0.05）。该方法可以发现以前未报道的有机和传统番茄生物活性成分的差异。
