Nutrition and Food Science Department, XaRTA, INSA, Pharmacy School, University of Barcelona, Barcelona, Spain.
J Agric Food Chem. 2013 Sep 4;61(35):8373-80. doi: 10.1021/jf401953y. Epub 2013 Aug 21.
The setup of a novel, rapid, and sensitive UHPLC-QqQ-MS method was described for the determination of phenolic compounds in tomatoes and tomato-based products (tomato sauce and juice). Phenolic compounds including hydroxybenzoic and hydroxycinnamic acid derivatives, flavonols, and flavanones were detected, separated, and quantified in a 3 min chromatographic run. The main advantages of the method include high analyte recovery (90.1-115%), low limit of detection (0.008-0.167 mg L(-1)) and quantification (0.01-0.83 mg L(-1)), good accuracy (85.6-115%), and precision (<15%). The detection of the phenolic compounds varied according to the physicochemical nature of the extracts, but generally low matrix-dependent suppression/enhancement effects were observed in all three matrices. The possibility to transfer easily the existing HPLC to the fast UHPLC methods is very attractive, and with minor modifications, the methodology described may be applied to the phenolic characterization of a broad range of plant and food matrices.
描述了一种新颖、快速和灵敏的 UHPLC-QqQ-MS 方法，用于测定番茄和番茄制品（番茄酱和汁）中的酚类化合物。在 3 分钟的色谱运行中，检测、分离和定量了包括羟基苯甲酸和羟基肉桂酸衍生物、类黄酮和黄烷酮在内的酚类化合物。该方法的主要优点包括高分析物回收率（90.1-115%）、低检测限（0.008-0.167 mg L(-1)) 和定量限（0.01-0.83 mg L(-1))）、良好的准确性（85.6-115%）和精密度（<15%）。酚类化合物的检测因提取物的物理化学性质而异，但在所有三种基质中都观察到低基质依赖性抑制/增强效应。将现有的 HPLC 轻松转换为快速 UHPLC 方法的可能性非常有吸引力，并且通过较小的修改，所描述的方法可以应用于广泛的植物和食品基质的酚类特征描述。
