Belostotskiĭ D A, Mil'shina N V, Anan'ev E V
Tsitol Genet. 1990 May-Jun;24(3):44-50.
High molecular weight "relic" DNA fraction can be separated from the bulk of barley DNA digested with different restriction enzymes by agarose gel electrophoresis. The majority of AluI-relic DNA clones contained barley simple sequence satellite DNA and other families of repetitive DNA. The clones representing HvRT family were sequenced. This family was found to be barley-specific. It is composed of tandemly arranged 118-bp monomers and presents in 7 x 10(5) copies in barley genome.
通过琼脂糖凝胶电泳，可以从用不同限制酶消化的大麦DNA主体中分离出高分子量的“遗迹”DNA片段。大多数AluI遗迹DNA克隆包含大麦简单序列卫星DNA和其他重复DNA家族。对代表HvRT家族的克隆进行了测序。发现这个家族是大麦特有的。它由串联排列的118个碱基对的单体组成，在大麦基因组中以7×10⁵个拷贝存在。
