羊驼（小羊驼）基因组中卫星I和II DNA的核苷酸序列。

Nucleotide sequence of satellite I and II DNA from alpaca (Lama pacos) genome.

作者信息

Sałuda-Gorgul A, Jaworski J, Greger J

机构信息

Department of Biochemistry I, Institute of Physiology and Biochemistry, Medical School of Lódź, Poland.

出版信息

Acta Biochim Pol. 1990;37(2):283-97.

PMID:2072986

Abstract

Agarose and polyacrylamide gel electrophoresis of a total alpaca (Lama pacos) DNA, digested with several restriction enzymes, revealed the presence of two tandemly organized repetitive DNA sequences, named Satellite I and Satellite II. Three Xhol-monomers from Satellite I DNA and two BspRI-monomers from Satellite II DNA were sequenced. As determined by dot hybridization analysis, the total alpaca DNA consists of 3.4% and 1.3% Satellites I and II, respectively. Computer search revealed no homology to any primate, rodent or mammalian sequences published in the Gen Bank Release 48.0 (February 1987) and the EMBL Bank Release 10 (December 1986).

摘要

用几种限制性内切酶消化的羊驼（小羊驼）总DNA进行琼脂糖和聚丙烯酰胺凝胶电泳，结果显示存在两个串联排列的重复DNA序列，分别命名为卫星I和卫星II。对卫星I DNA的三个Xhol单体和卫星II DNA的两个BspRI单体进行了测序。通过点杂交分析确定，羊驼总DNA中卫星I和卫星II分别占3.4%和1.3%。计算机检索未发现与1987年2月发布的Gen Bank 48.0版和1986年12月发布的EMBL Bank 10版中公布的任何灵长类、啮齿动物或哺乳动物序列具有同源性。