Aleksandrova A Iu, Vasil'ev Iu M, Domnina L V, Liubimov A V, Svitkina T M
Tsitologiia. 1990;32(6):633-40.
Formation of extracellular matrix structures in cultures of rat liver epithelial nontransformed cell line IAR2 was studied with antisera to fibronectin, laminin and type IV collagen by immunofluorescence and immunoelectron microscopy of platinum replicas. Fibronectin formed peripheral spots of variable size some of which outlined free cell edges, as well as fibrils located towards the center of single cells or of cellular islands. Similarly distributed structures were seen in isolated matrices. Codistribution of fibronectin and actin was observed only for the peripheral line of fibronectin spots and marginal circular actin bundle. Basement membrane components. laminin and type IV collagen, formed mainly spots of variable size predominantly beneath the cell or each cell in an island. Occasional fibrils were seen also. Essentially the same results were obtained by immunofluorescence and immunogold electron microscopy. Cytochalasin D treated cells displayed spots of both fibronectin and laminin. The relevance of previously postulated receptor-mediated assembly of extracellular matrix structures to the epithelial cells is discussed.
通过对铂复制品进行免疫荧光和免疫电子显微镜检查，利用针对纤连蛋白、层粘连蛋白和IV型胶原的抗血清，研究了大鼠肝脏上皮非转化细胞系IAR2培养物中细胞外基质结构的形成。纤连蛋白形成大小不一的周边斑点，其中一些勾勒出游离细胞边缘，以及朝向单细胞或细胞岛中心的纤维。在分离的基质中也观察到类似分布的结构。仅在纤连蛋白斑点的周边线和边缘圆形肌动蛋白束处观察到纤连蛋白和肌动蛋白的共分布。基底膜成分，层粘连蛋白和IV型胶原，主要形成大小不一的斑点，主要在细胞下方或岛中的每个细胞下方。偶尔也可见纤维。免疫荧光和免疫金电子显微镜获得了基本相同的结果。用细胞松弛素D处理的细胞显示出纤连蛋白和层粘连蛋白的斑点。讨论了先前假定的受体介导的细胞外基质结构组装与上皮细胞的相关性。
