Foidart J M, Berman J J, Paglia L, Rennard S, Abe S, Perantoni A, Martin G R
Lab Invest. 1980 May;42(5):525-32.
We have investigated the ability of ARL-6 cells, a cell line derived from rat liver, to synthesize various collagens and two glycoproteins of the extracellular matrix, fibronectin, and laminin. Using immunofluorescence, we detected types I, II, and IV collagen plus laminin and fibronectin. Antibodies to types I and III collagen and to fibronectin were associated with most cells and showed a similar distribution. Type IV collagen and laminin were found in thin filaments associated with a small proportion of the cells. Chemical studies showed that ARL-6 cells synthesize predominantly types I and III collagens. The level of collagen synthesis was greatly affected by the presence of exogenous fibronectin added to the cells in the media. Cells maintained in fibronectin-free serum synthesized much less collagen. These studies indicate that liver-derived cells can synthesize a variety of connective tissue proteins and that collagen synthesis by these cells is enhanced by the presence of fibronectin.
我们研究了源自大鼠肝脏的ARL - 6细胞系合成各种胶原蛋白以及细胞外基质的两种糖蛋白（纤连蛋白和层粘连蛋白）的能力。利用免疫荧光技术，我们检测到了I型、II型和IV型胶原蛋白以及层粘连蛋白和纤连蛋白。抗I型和III型胶原蛋白以及抗纤连蛋白的抗体与大多数细胞相关，并呈现出相似的分布。IV型胶原蛋白和层粘连蛋白存在于与一小部分细胞相关的细丝中。化学研究表明，ARL - 6细胞主要合成I型和III型胶原蛋白。培养基中添加的外源性纤连蛋白的存在对胶原蛋白的合成水平有很大影响。在不含纤连蛋白的血清中培养的细胞合成的胶原蛋白要少得多。这些研究表明，源自肝脏的细胞能够合成多种结缔组织蛋白，并且这些细胞的胶原蛋白合成会因纤连蛋白的存在而增强。
