School of Chemical and Biological Engineering, Seoul National University, Seoul 151-747, Korea.
Molecules. 2012 Mar 1;17(3):2474-90. doi: 10.3390/molecules17032474.
Potential utilization of proteins for early detection and diagnosis of various diseases has drawn considerable interest in the development of protein-based multiplex detection techniques. Among the various techniques for high-throughput protein screening, optically-encoded beads combined with fluorescence-based target monitoring have great advantages over the planar array-based multiplexing assays. This review discusses recent developments of analytical methods of screening protein molecules on microbead-based platforms. These include various strategies such as barcoded microbeads, molecular beacon-based techniques, and surface-enhanced Raman scattering-based techniques. Their applications for label-free protein detection are also addressed. Especially, the optically-encoded beads such as multilayer fluorescence beads and SERS-encoded beads are successful for generating a large number of coding.
蛋白质在早期检测和诊断各种疾病方面的潜在应用，引起了人们对基于蛋白质的多重检测技术的极大兴趣。在高通量蛋白质筛选的各种技术中，与基于平面阵列的多重分析相比，光学编码珠与基于荧光的靶标监测相结合具有很大的优势。本文综述了基于微珠平台筛选蛋白质分子的分析方法的最新进展。这些策略包括条形码微珠、分子信标技术和基于表面增强拉曼散射的技术等。还讨论了它们在无标记蛋白质检测中的应用。特别是多层荧光珠和 SERS 编码珠等光学编码珠在产生大量编码方面非常成功。
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