College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang, China.
J Nat Med. 2013 Jan;67(1):207-11. doi: 10.1007/s11418-012-0648-9. Epub 2012 Mar 2.
Rhizoma Smilacis Glabrae (RSG) is a commonly used herbal material in functional food and Traditional Chinese Medicine. A HPLC chromatographic fingerprint was developed for its quality control and species differentiation. Nine peaks were found in the chromatogram of RSG and all these peaks were identified by diode array detection and electrospray ionization-MS/MS: 5-O-caffeoylshikimic acid, taxifolin, engeletin, isoengeletin, trans-resveratrol, astilbin and its three stereoisomers. Six of these constituents were consistently found in 18 batches of samples. The standard fingerprint of RSG was generated by mean simulation of all tested samples. Using the standard fingerprint, RSG could be easily differentiated from Rhizoma Smilacis Chinae and Rhizoma Heterosmilacis, the two species that can be confused with RSG.
土茯苓（Rhizoma Smilacis Glabrae，RSG）是一种常用于功能性食品和中药的草药材料。为了对其进行质量控制和物种鉴别，建立了一种高效液相色谱指纹图谱。在 RSG 的色谱图中发现了 9 个峰，通过二极管阵列检测和电喷雾电离-质谱/质谱鉴定了所有这些峰：5-O-咖啡酰莽草酸、杨梅素、恩格莱汀、异恩格莱汀、反式白藜芦醇、阿魏酸及其三种立体异构体。这 6 种成分在 18 批样品中均一致存在。通过对所有测试样品的平均模拟生成了 RSG 的标准指纹图谱。使用标准指纹图谱，RSG 可以很容易地与容易与 RSG 混淆的两种物种菝葜（Rhizoma Smilacis Chinae）和异叶菝葜（Rhizoma Heterosmilacis）区分开来。
