Aomori Prefectural Institute of Public Health and Environment, 1-1-1 Higashitsukurimichi, Aomori-shi, Aomori 030-8566, Japan.
Kumamoto Prefectural Institute of Public Health and Environmental Sciences, 1240-1 Kurisaki-machi, Uto-shi, Kumamoto 869-0425, Japan.
J Med Microbiol. 2012 Jun;61(Pt 6):820-829. doi: 10.1099/jmm.0.041137-0. Epub 2012 Mar 1.
This study performed a detailed genetic analysis of the glycoprotein (G) gene of respiratory syncytial virus (RSV) detected in 50 Japanese children with acute respiratory infection (ARI) in the 2009/2010 season. A phylogenetic tree constructed by the neighbour-joining method showed that 34 and 16 of the RSV strains could be classified into subgroups A and B, respectively. Strains belonging to subgroups A and B were further subdivided into GA2 and BA, respectively. The nucleotide and deduced amino acid sequence identities were relatively high among these strains (>90%). The deduced amino acid sequences implied that a relatively high frequency of amino acid substitutions occurred in the C-terminal 3rd hypervariable region of the G protein in these strains. In addition, some positively selected sites were estimated. The results suggest that RSV with genotypes GA2 and BA was associated with ARI in Japanese children in 2009/2010.
本研究对 2009/2010 季节 50 名日本急性呼吸道感染（ARI）儿童呼吸道合胞病毒（RSV）的糖蛋白（G）基因进行了详细的遗传分析。通过邻接法构建的系统进化树显示，34 株和 16 株 RSV 株可分别分为亚组 A 和 B。属于亚组 A 和 B 的株进一步分为 GA2 和 BA。这些株之间的核苷酸和推导的氨基酸序列同一性相对较高（>90%）。推导的氨基酸序列表明，这些株的 G 蛋白 C 末端第 3 个高变区的氨基酸替换频率相对较高。此外，还估计了一些正选择位点。结果表明，2009/2010 年日本儿童的 ARI 与 GA2 和 BA 基因型 RSV 有关。
