National Hospital Organization Yokohama Medical Center, Kanagawa 245-8575, Japan.
Jpn J Infect Dis. 2010 Nov;63(6):393-400.
We performed the genotyping and phylogenetic analysis of respiratory syncytial virus (RSV) isolated from 17 infants with bronchiolitis in Kanagawa Prefecture, Japan in 2005 and 2006. The major genes in these samples (attachment [G] glycoprotein gene, fusion [F] protein gene, and nucleoprotein [N] gene) were sequenced and analyzed genetically. Phylogenetic analysis of these genes revealed that 7 and 10 strains could be classified into subgroups A and B, respectively. Phylogenetic analysis of the G gene revealed that the subgroup A and B strains were unique genotypes GA2 and BA, respectively. Moreover, the amino acid sequences for these genotypes suggested a relatively high frequency of amino acid substitutions in the G and F proteins in these strains, whereas the N protein was highly homologous. These results suggest that RSV genotypes GA2 and BA may be associated with bronchiolitis in the cases studied here.
我们对 2005 年和 2006 年在日本神奈川县的 17 名毛细支气管炎婴儿分离的呼吸道合胞病毒（RSV）进行了基因分型和系统发育分析。对这些样本中的主要基因（附着[G]糖蛋白基因、融合[F]蛋白基因和核蛋白[N]基因）进行了测序和遗传分析。这些基因的系统发育分析表明，7 株和 10 株病毒可分别归入亚组 A 和 B。G 基因的系统发育分析表明，亚组 A 和 B 株分别为独特的 GA2 和 BA 基因型。此外，这些基因型的氨基酸序列提示这些病毒株的 G 和 F 蛋白中氨基酸取代的相对高频，而 N 蛋白则高度同源。这些结果表明，在本研究中，RSV 基因型 GA2 和 BA 可能与毛细支气管炎有关。
