培养中发育海马神经元活体成像的一般注意事项。
General considerations for live imaging of developing hippocampal neurons in culture.
作者信息
Kaech Stefanie, Huang Chun-Fang, Banker Gary
出版信息
Cold Spring Harb Protoc. 2012 Mar 1;2012(3):312-8. doi: 10.1101/pdb.ip068221.
Abstract
Dissociated cell cultures of the rodent hippocampus have become a standard model for studying many facets of neural development, including the development of polarity, axonal and dendritic growth, and synapse formation. The cultures are quite homogeneous--∼90% of the cells are pyramidal neurons--and it is relatively easy to express green fluorescent protein (GFP)-tagged proteins by transfection. This article describes the cultures and the key features of the system used to image them. It also includes suggestions on labeling cells with GFP-tagged proteins. It concludes with a discussion of the advantages and disadvantages of this culture system.
摘要
啮齿动物海马体的解离细胞培养已成为研究神经发育诸多方面的标准模型，包括极性发育、轴突和树突生长以及突触形成。这些培养物相当均匀，约90%的细胞是锥体神经元，并且通过转染表达绿色荧光蛋白（GFP）标记的蛋白质相对容易。本文描述了这些培养物以及用于对其成像的系统的关键特征。它还包括用GFP标记的蛋白质标记细胞的建议。最后讨论了这种培养系统的优缺点。
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