Institut de Systématique, Évolution, Biodiversité (ISYEB), Muséum national d'Histoire naturelle, CNRS, Sorbonne Université, EPHE, Université des Antilles Pointe-à-Pitre, Guadeloupe, France.
CIRAD, UMR ASTRE, Petit-Bourg, Guadeloupe, France.
PLoS One. 2022 Aug 30;17(8):e0273668. doi: 10.1371/journal.pone.0273668. eCollection 2022.
Wolbachia Hertig, 1936 is an intracellular bacterial symbiont colonizing many arthropods. Of the studies done on the bacteria present in the superfamily Gerroidea Leach, 1815, no report of Wolbachia infection had yet been made. Thus, we checked the presence of Wolbachia in six Gerroidea species which colonize tropical aquatic environments by PCR using wsp primer set before sequencing and phylogenetic analyses. Insects were collected in the marine fringe of mangroves, in river estuaries, in swampy mangroves, and in ponds from Guadeloupe islands (Caribbean). Two new strains of Wolbachia were detected in these Gerroidea. They were named wLfran and wRmang. The wsp sequences suggest that the strains belong to the already described E supergroup or similar. wLfran is present in Limnogonus franciscanus Stål, 1859 and Rheumatobates trinitatis (China, 1943) while wRmang appears to be present exclusively in R. mangrovensis (China, 1943). Three other species were analysed, but did not appear to be infected: Brachymetra albinerva (Amyot & Serville, 1843), Halobates micans Eschscheltz, 1822, and Microvelia pulchella Westwood, 1834. The results presented here highlight for the first time the presence of new intracellular Wolbachia strains in Gerroidea colonising tropical aquatic environments like mangrove habitats from inlands to sea shore.
沃尔巴克氏体（Wolbachia Hertig，1936）是一种存在于许多节肢动物体内的细胞内共生细菌。在对 Gerroidea Leach，1815 超科中存在的细菌进行的研究中，尚未报道过沃尔巴克氏体感染的情况。因此，我们通过 PCR 使用 wsp 引物组在测序和系统发育分析之前，检查了六种栖息在热带水生环境中的 Gerroidea 物种中沃尔巴克氏体的存在情况。昆虫是在红树林的海洋边缘、河口、沼泽红树林和瓜德罗普岛（加勒比海）的池塘中收集的。在这些 Gerroidea 中检测到两种新的沃尔巴克氏体菌株。它们被命名为 wLfran 和 wRmang。wsp 序列表明，这些菌株属于已经描述的 E 超组或类似的菌株。wLfran 存在于 Limnogonus franciscanus Stål，1859 和 Rheumatobates trinitatis（中国，1943）中，而 wRmang 似乎仅存在于 R. mangrovensis（中国，1943）中。另外三种物种也进行了分析，但似乎没有被感染：Brachymetra albinerva（Amyot & Serville，1843），Halobates micans Eschscheltz，1822 和 Microvelia pulchella Westwood，1834。这里呈现的结果首次强调了在栖息在红树林等热带水生环境中的 Gerroidea 中存在新的细胞内沃尔巴克氏体菌株。
