Chen Xiao-yan, Zhou Li, Zheng Fei-yun
Department of Gynecology and Obstetrics, First Affiliated Hospital of Wenzhou Medical College, Zhejiang.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2011 Dec;31(12):1651-6.
To study the proteomics of Pinellia pedatisecta Schott total protein on human ovarian cancer SKOV3 cells.
SKOV3 cells were in vitro cultured. The growth inhibition of SKOV3 cells in the logarithmic phase with different concentrations of Pinellia pedatisecta Schott (0, 0.05, 0.10, 0.20, 0.30, 0.40, and 0.50 mg/mL) at different time points (24, 48, 72, and 96 h) was analyzed by CCK-8 colorimetry. The total protein was extracted after adding 0.296 mg/mL Pinellia pedatisecta Schott protein for 48 h. The protein with differential expressions was screened out using two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS).
The protein of Pinellia pedatisecta Schott (at 0.10 - 0.50 mg/mL) could significantly inhibit the growth of SKOV3 cells in a time- and dose-dependent manner (P < 0.05, P < 0.01). After analyzed by 2-DE and MALDI-TOF-MS, 43 differential protein dots were successfully identified (21 up-regulated and 22 down-regulated), including alpha-enolase 1, eukaryotic initiation factor 3alpha, cyclophilin B, and so on.
Protein of Pinellia pedatisecta Schott could significantly inhibit the growth of SKOV3 cells, and lead to the proteomics changes of SKOV3 cell strain. They might be correlated with its anti-tumor mechanisms.
研究掌叶半夏总蛋白对人卵巢癌SKOV3细胞的蛋白质组学影响。
体外培养SKOV3细胞，采用CCK-8比色法分析不同浓度掌叶半夏（0、0.05、0.10、0.20、0.30、0.40和0.50 mg/mL）在不同时间点（24、48、72和96 h）对对数期SKOV3细胞的生长抑制作用。加入0.296 mg/mL掌叶半夏蛋白作用48 h后提取总蛋白，运用二维凝胶电泳（2-DE）和基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）筛选差异表达蛋白。
掌叶半夏蛋白（0.10 - 0.50 mg/mL）能显著抑制SKOV3细胞生长，呈时间和剂量依赖性（P < 0.05，P < 0.01）。经2-DE和MALDI-TOF-MS分析，成功鉴定出43个差异蛋白点（21个上调，22个下调），包括α-烯醇化酶1、真核起始因子3α、亲环素B等。
掌叶半夏蛋白可显著抑制SKOV3细胞生长，并导致SKOV3细胞株蛋白质组学变化，可能与其抗肿瘤机制相关。
