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[干旱及复水条件下叶面喷施茉莉酸甲酯对烤烟幼苗叶片叶绿素荧光特性的影响]

[Effects of foliar spraying methyl jasmonate on leaf chlorophyll fluorescence characteristics of flue-cured tobacco seedlings under drought and re-watering].

作者信息

Jin Wei-Wei, Wang Yan, Zhang Hui-Hui, Jiao Zhi-Li, Wang Peng, Li Xin, Yue Bing-Bing, Sun Guang-Yu

机构信息

College of Life Science, Northeast Forestry University, Harbin 150040, China.

出版信息

Ying Yong Sheng Tai Xue Bao. 2011 Dec;22(12):3157-62.

Abstract

Taking the flue-cured tobacco variety of "Longjiang 911" from Heilongjiang Province of Northeast China as test material, a pot experiment was conducted to study the effects of foliar spraying different concentration methyl jasmonate (MeJA) on the seedlings leaf chlorophyll content and chlorophyll fluorescence characteristics during the transplantation stage under drought and re-watering. Under drought condition, the leaf chlorophyll content, maximum fluorescence (F(m)), potential activities of PSII (F(v)/F(o)), maximum photochemical efficiency (F(v)/F(m)), actual photochemical efficiency (psi(PSII)), apparent electron transport rate (ETR), and photochemical quenching (q(P)) decreased, but the minimal fluorescence (F(o)) and non-photochemical quenching (q(N)) increased. Foliar spraying 0.2 and 0.5 mmol x L(-1) of MeJA had obvious positive effects in mitigating the decrease of F(v)/F(m), F(v)/F(o), phi(PSII), ETR, and q(P) and the increase of q(N) under drought stress, while spraying 1.0 mmol x L(-1) of MeJA had lesser effects. After re-watering, all the leaf chlorophyll fluorescence indices had obvious recovery, and spraying MeJA made the indices more close to the original levels before drought. It was suggested that foliar spraying MeJA could alleviate the degradation of chlorophyll and play definite role in protecting the PSII under drought stress, decrease the damage of drought stress on the seedlings, promote the rapid MeJA recovery of chlorophyll fluorescence parameters after re-watering, and thus, ensure the regrowth of flue-cured tobacco seedlings.

摘要

以中国东北黑龙江省的烤烟品种“龙江911”为试验材料,进行盆栽试验,研究在干旱和复水条件下,移栽期叶面喷施不同浓度茉莉酸甲酯(MeJA)对烟苗叶片叶绿素含量和叶绿素荧光特性的影响。干旱条件下,叶片叶绿素含量、最大荧光(F(m))、PSII潜在活性(F(v)/F(o))、最大光化学效率(F(v)/F(m))、实际光化学效率(psi(PSII))、表观电子传递速率(ETR)和光化学猝灭(q(P))降低,而最小荧光(F(o))和非光化学猝灭(q(N))增加。叶面喷施0.2和0.5 mmol·L(-1)的MeJA对缓解干旱胁迫下F(v)/F(m)、F(v)/F(o)、phi(PSII)、ETR和q(P)的降低以及q(N)的增加有明显的积极作用,而喷施1.0 mmol·L(-1)的MeJA效果较差。复水后,所有叶片叶绿素荧光指标均有明显恢复,喷施MeJA使指标更接近干旱前的原始水平。表明叶面喷施MeJA能缓解干旱胁迫下叶绿素的降解,对保护PSII有一定作用,减轻干旱胁迫对烟苗的伤害,促进复水后叶绿素荧光参数的快速恢复,从而保证烤烟烟苗的再生长。

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