Johnson James R, Kuskowski Michael A, Owens Krista, Clabots Connie, Singer Randall S
Veterans Affairs Medical Center, Minneapolis, MN, USA.
Vet Microbiol. 2009 Apr 14;136(1-2):108-14. doi: 10.1016/j.vetmic.2008.10.006. Epub 2008 Oct 15.
The origins and virulence potential of fluoroquinolone-resistant (FQ-R) Escherichia coli from dogs with urinary tract infection (UTI) are undefined. Therefore, fluoroquinolone-resistant (n=38) or susceptible (n=62) E. coli urine isolates from dogs with UTI were characterized for phylogenetic group (A, B1, B2, D) and 61 virulence-associated genes by multiplex PCR, then were compared according to these characteristics. Compared with fluoroquinolone-susceptible (FQ-S) isolates, the fluoroquinolone-resistant isolates exhibited significantly lower prevalences for most virulence genes studied (albeit higher prevalences for several, including iutA: aerobactin receptor), significantly fewer virulence genes per isolate, and shifts away from virulence-associated group B2. Nonetheless, 26% of fluoroquinolone-resistant isolates qualified as extraintestinal pathogenic E. coli (ExPEC), suggesting possible human virulence potential. The findings call into question whether the fluoroquinolone-resistant E. coli encountered in dogs arise through conversion of fluoroquinolone-susceptible canine resident strains to resistance, or instead are imported from an external source. They also identify dogs as a possible reservoir of drug-resistant ExPEC for transmission to other pets and humans.
来自患有尿路感染（UTI）犬类的耐氟喹诺酮（FQ-R）大肠杆菌的起源和毒力潜力尚不明确。因此，通过多重PCR对来自患有UTI犬类的耐氟喹诺酮（n = 38）或敏感（n = 62）大肠杆菌尿液分离株进行系统发育组（A、B1、B2、D）和61个毒力相关基因的特征分析，然后根据这些特征进行比较。与氟喹诺酮敏感（FQ-S）分离株相比，耐氟喹诺酮分离株在所研究的大多数毒力基因中表现出显著较低的流行率（尽管包括iutA：气杆菌素受体在内的几个基因流行率较高），每个分离株的毒力基因显著较少，并且偏离了与毒力相关的B2组。尽管如此，26%的耐氟喹诺酮分离株符合肠外致病性大肠杆菌（ExPEC）的标准，表明可能具有对人类的毒力潜力。这些发现使人质疑在犬类中遇到的耐氟喹诺酮大肠杆菌是通过氟喹诺酮敏感的犬类常驻菌株转化为耐药性产生的，还是从外部来源引入的。它们还将犬类确定为耐药ExPEC的可能储存宿主，可传播给其他宠物和人类。
