Departamento de Química, Pontifícia Universidade Católica do Rio de Janeiro, Rio de Janeiro 22451-900, Brazil.
Spectrochim Acta A Mol Biomol Spectrosc. 2013 Jan 1;100:51-8. doi: 10.1016/j.saa.2012.01.043. Epub 2012 Feb 9.
A phosphorimetric method was developed to enable the determination of enrofloxacin using photochemical derivatization which was used to both improve detection limits and to minimize the uncertainty of measurements. Phosphorescence was induced on cellulose containing TlNO(3). Absolute limit of detection at the ng range and linear analytical response over three orders of magnitude were achieved. A metrological study was made to obtain the combined uncertainty value and to identify that the precision was mainly affected by the changing of substrates when measuring the signal from each replicate. Pharmaceutical formulations containing enrofloxacin were successfully analyzed by the method and the results were similar to the ones achieved using a HPLC method. A solid phase extraction on an acrylic polymer was optimized to separate enrofloxacin from interferents such as diclofenac and other components from biological matrices, which allowed the successful use of the method in urine analysis.
建立了一种利用光化学衍生化的荧光光度法来测定恩诺沙星的方法，该方法既提高了检测限，又最小化了测量的不确定度。在含有 TlNO(3) 的纤维素上诱导磷光。在纳克范围内达到了绝对检测限，在三个数量级范围内实现了线性分析响应。进行了一项计量学研究以获得合并不确定度值，并确定在测量每个重复信号时，主要受基质变化影响的是精度。该方法成功地分析了含有恩诺沙星的药物制剂，其结果与使用 HPLC 方法获得的结果相似。在丙烯聚合物上进行了固相萃取，以将恩诺沙星与干扰物（如双氯芬酸）和生物基质中的其他成分分离，这使得该方法能够成功地用于尿液分析。
