Department of Microbiology and Cell Science, University of Florida, Gainesville, FL 32611-0700, USA.
Curr Opin Microbiol. 2012 Jun;15(3):351-6. doi: 10.1016/j.mib.2012.02.002. Epub 2012 Mar 1.
Archaea display amazing physiological properties that are of interest to understand at the molecular level including the ability to thrive at extreme environmental conditions, the presence of novel metabolic pathways (e.g. methanogenesis, methylaspartate cycle) and the use of eukaryotic-like protein machineries for basic cellular functions. Coupling traditional genetic and biochemical approaches with advanced technologies, such as genomics and proteomics, provides an avenue for scientists to discover new aspects related to the molecular physiology of archaea. This review emphasizes the unusual properties of archaeal proteomes and how high-throughput and specialized mass spectrometry-based proteomic studies have provided insight into the molecular properties of archaeal cells.
古菌表现出令人惊讶的生理特性，这些特性在分子水平上引起了人们的兴趣，包括在极端环境条件下茁壮成长的能力、新型代谢途径的存在（例如产甲烷作用、甲基天冬氨酸循环）以及真核样蛋白机器在基本细胞功能中的应用。将传统的遗传和生化方法与先进技术（如基因组学和蛋白质组学）相结合，为科学家提供了一条发现与古菌分子生理学相关的新方面的途径。本综述强调了古菌蛋白质组的独特特性，以及高通量和专门的基于质谱的蛋白质组学研究如何深入了解古菌细胞的分子特性。
