Analytical Technologies Department, Centro de Investigación Lilly SA, Avda de la Industria 30, 28108-Alcobendas, Madrid, Spain.
Drug Discov Today. 2012 Apr;17(7-8):396-404. doi: 10.1016/j.drudis.2012.02.008. Epub 2012 Feb 23.
Capillary electrophoresis (CE) is an analytical technique based on the separation of the analytes within a capillary owing to their different electrophoretic mobilities. It is widely used in pharmaceutical analyses owing to its versatility and high separation power. However, its penetration into the drug discovery scene has been relatively limited until recent years. Several factors have contributed to this low implementation, including the maturity of liquid chromatography, the scarcity of experienced CE practitioners, and certain limitations intrinsic to the technique. Recently, instrumental improvements and the growing demand for analytical information have lead to a continuously expanding range of routine electrophoretic applications throughout pharmaceutical discovery and development. In this article we review CE fundamentals, review well-established CE methodologies in drug discovery of small molecules and discuss trends that, in our opinion, might emerge in the coming years.
毛细管电泳（CE）是一种基于分析物在毛细管内由于其不同电泳迁移率而分离的分析技术。由于其多功能性和高分离能力，它在药物分析中得到了广泛应用。然而，直到近年来，它在药物发现领域的应用才相对有限。造成这种低实施率的因素包括：液相色谱的成熟度、经验丰富的毛细管电泳从业者的稀缺性以及该技术固有的某些局限性。最近，仪器的改进和对分析信息的需求不断增长，导致毛细管电泳在药物发现和开发中的常规应用范围不断扩大。本文综述了 CE 的基本原理，综述了小分子药物发现中成熟的 CE 方法，并讨论了我们认为在未来几年可能出现的趋势。
