Institute of Medicinal and Pharmaceutical Chemistry, Technische Universität Braunschweig, 38106 Braunschweig, Germany.
Departamento de Ciências Naturais, Campus Dom Bosco, Universidade Federal de São João del-Rei (UFSJ), Praça Dom Helvécio 74, Fábricas, São João del-Rei 36301-160, Minas Gerais, Brazil.
Molecules. 2021 May 11;26(10):2841. doi: 10.3390/molecules26102841.
This work reviews the literature of chiral capillary electrokinetic chromatography from January 2016 to March 2021. This is done to explore the state-of-the-art approach and recent developments carried out in this field. The separation principle of the technique is described and supported with simple graphical illustrations, showing migration under normal and reversed polarity modes of the separation voltage. The most relevant applications of the technique for enantioseparation of drugs and other enantiomeric molecules in different fields using chiral selectors in single, dual, or multiple systems are highlighted. Measures to improve the detection sensitivity of chiral capillary electrokinetic chromatography with UV detector are discussed, and the alternative aspects are explored, besides special emphases to hyphenation compatibility to mass spectrometry. Partial filling and counter migration techniques are described. Indirect identification of the separated enantiomers and the determination of enantiomeric migration order are mentioned. The application of Quality by Design principles to facilitate method development, optimization, and validation is presented. The elucidation and explanation of chiral recognition in molecular bases are discussed with special focus on the role of molecular modeling.
本工作综述了 2016 年 1 月至 2021 年 3 月期间的手性毛细管电泳文献。目的是探索该领域的最新研究进展和现状。本文描述了该技术的分离原理，并辅以简单的图形说明，展示了在分离电压的常规和反转极性模式下的迁移情况。重点介绍了该技术在手性选择剂在单、双或多体系中对不同领域药物和其他对映异构体分子的对映体分离的最相关应用。讨论了提高手性毛细管电泳与紫外检测器检测灵敏度的措施，并探讨了替代方法，除了特别强调与质谱的连接兼容性。部分填充和逆流迁移技术也进行了描述。提到了分离对映异构体的间接鉴定和对映体迁移顺序的确定。介绍了将质量设计原则应用于促进方法开发、优化和验证。讨论了在分子基础上阐明和解释手性识别的问题，特别关注分子建模的作用。
