Department of Anaesthesiology and Pain Medicine, College of Medicine, Korea University, Seoul, Republic of Korea.
Clin Hemorheol Microcirc. 2012;51(4):287-92. doi: 10.3233/CH-2012-1535.
This in vitro study investigated time-related effects of propofol at the plasma concentrations required for sedation and general anaesthesia, on RBC aggregation, deformability, and morphology. Blood containing propofol at plasma concentrations of 0, 2 and 4 μg ml-1 was incubated in a water bath at 37°C for 1, 2, or 4 hours. RBC elongation indices (EIs) and aggregation indices (AIs), which represent RBC deformability and aggregation, respectively, were measured. Also, RBC morphological indices (MIs), which represent RBC morphology, were calculated. EIs and AIs were similar at propofol concentrations of 0, 2, or 4 μg ml-1 after 1, 2, or 4 hours of incubation. MIs at propofol plasma concentrations 0 or 2 μg ml-1 were similar after 1, 2, and 4 hours of incubation, however, MI at a propofol concentration of 4 μg ml-1 after 4 hours of incubation was higher than its value after 1 or 2 hours of incubation. No significant difference was observed between MIs at propofol plasma concentrations 0, 2, or 4 μg ml-1 after 1, 2, and 4 hours of incubation. At clinical doses, propofol has no direct effects on RBC deformability, aggregation, or morphology over a 4 hours incubation period.
本体外研究探讨了在镇静和全身麻醉所需的血浆浓度下，丙泊酚对 RBC 聚集、变形和形态的时间相关影响。将含有丙泊酚的血液置于 37°C 的水浴中孵育 1、2 或 4 小时，血浆浓度分别为 0、2 和 4 μg ml-1。分别测量代表 RBC 变形性和聚集性的 RBC 伸长指数 (EI) 和聚集指数 (AI)，并计算代表 RBC 形态的 RBC 形态指数 (MI)。在孵育 1、2 或 4 小时后，丙泊酚浓度为 0、2 或 4 μg ml-1 时的 EI 和 AI 相似。在孵育 1、2 和 4 小时后，丙泊酚血浆浓度为 0 或 2 μg ml-1 时的 MI 相似，但丙泊酚浓度为 4 μg ml-1 时，在孵育 4 小时后的 MI 值高于孵育 1 或 2 小时后的 MI 值。在孵育 1、2 和 4 小时后，丙泊酚血浆浓度为 0、2 或 4 μg ml-1 时的 MI 无显著差异。在 4 小时孵育期间，临床剂量的丙泊酚对 RBC 变形性、聚集或形态没有直接影响。
