Fehmann H C, Göke R, Göke B, Arnold R
Department of Internal Medicine, Philipps-University Marburg, Fed. Rep. Germany.
Z Gastroenterol. 1990 Jul;28(7):348-52.
The priming effect of carbachol on glucose-, arginine- and glucose plus GLP-1 (7-36)amide induced insulin secretion was investigated. The isolated rat pancreas was perfused in vitro during a basal period of 10 min with Krebs-Ringer-bicarbonate buffer containing 2.8 mmol/l glucose. This medium was then supplemented with carbachol (10,1.0.1 mumol/l, respectively). After an additional 10 min period at 2.8 mmol/l glucose insulin secretion was stimulated for 44 min with 10 mmol/l glucose, or glucose plus GLP-1 (7-36)amide (0.5 nmol/l), or arginine (10 mmol/l). Pretreatment with carbachol resulted in a concentration dependent sensitization of B-cells to a consecutive glucose load (10 mmol/l). Both phases of the biphasic insulin secretory response were significantly enhanced. Priming experiments followed by a subsequent combined glucose / GLP-1 (7-36)amide or arginine stimulation were performed with 10 mumol/l carbachol. Prior exposure of the pancrease to carbachol enhanced the glucose / GLP-1 (7-36)amide induced insulin release, but left the arginine stimulated secretion unaltered. Our data suggest that in the regulation of postprandial insulin release cholinergic sensitizing effects might be involved which are mediated by muscarinic receptors.
研究了卡巴胆碱对葡萄糖、精氨酸以及葡萄糖加胰高血糖素样肽-1（7-36）酰胺诱导的胰岛素分泌的启动作用。在基础期10分钟内，用含2.8 mmol/L葡萄糖的 Krebs-Ringer-碳酸氢盐缓冲液对离体大鼠胰腺进行体外灌注。然后分别向该培养基中添加卡巴胆碱（10、1.0、0.1 μmol/L）。在2.8 mmol/L葡萄糖条件下再持续10分钟后，用10 mmol/L葡萄糖、或葡萄糖加胰高血糖素样肽-1（7-36）酰胺（0.5 nmol/L）、或精氨酸（10 mmol/L）刺激胰岛素分泌44分钟。用卡巴胆碱预处理导致β细胞对随后的葡萄糖负荷（10 mmol/L）呈浓度依赖性致敏。双相胰岛素分泌反应的两个阶段均显著增强。用10 μmol/L卡巴胆碱进行启动实验，随后进行葡萄糖 / 胰高血糖素样肽-1（7-36）酰胺或精氨酸联合刺激。胰腺预先暴露于卡巴胆碱可增强葡萄糖 / 胰高血糖素样肽-1（7-36）酰胺诱导的胰岛素释放，但精氨酸刺激的分泌未改变。我们的数据表明，在餐后胰岛素释放的调节中可能涉及由毒蕈碱受体介导的胆碱能致敏作用。
