Anhui Key Laboratory of Chemo-Biosensing, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2012 Jun;91:295-300. doi: 10.1016/j.saa.2012.02.019. Epub 2012 Feb 10.
A novel flow injection nanocrystals (NCs) chemiluminescence (CL) analysis method has been established for the determination of estradiol, estriol and estrone based on the enhancement of CdTe NCs-KMnO(4) CL reaction catalyzed by sodium hexametaphosphate. Glutathione (GSH)-capped CdTe nanocrystals were synthesized in aqueous medium, and the CdTe NCs emitted at around 555 nm was selected as the light emitter in CdTe NCs-KMnO(4) chemiluminescence (CL) system. It has been found that sodium hexametaphosphate (SHMP) enhanced the CL of the CdTe NCs-KMnO(4) system and estrogens increased these CL signals again in near neutral solution. UV-visible spectra, photoluminescence (PL) spectra, transmission electron microscopy (TEM) and CL spectra were used to characterize CdTe nanoparticles and investigate the mechanism of the CL reaction. On the basis of the enhancement, a novel flow-injection CL method has been established for the determination of estrogens. Under the optimum experimental conditions, three linear relationships were obtained. The method described is simple, sensitive, and has been successfully utilized for the determination of estrogens in tap water samples.
建立了一种基于六偏磷酸钠增敏 CdTe 纳米晶-高锰酸钾（KMnO4）化学发光（CL）反应测定雌二醇、雌三醇和雌酮的新流动注射纳米晶（NCs）CL 分析方法。在水相介质中合成了谷胱甘肽（GSH）封端的 CdTe 纳米晶，选择发射波长约为 555nm 的 CdTe NCs 作为 CdTe NCs-KMnO4 CL 体系中的发光体。实验发现，六偏磷酸钠（SHMP）增强了 CdTe NCs-KMnO4 体系的 CL，而雌激素在近中性溶液中再次增强了这些 CL 信号。紫外-可见光谱、光致发光（PL）光谱、透射电子显微镜（TEM）和 CL 光谱用于表征 CdTe 纳米粒子并研究 CL 反应的机制。在此增强基础上，建立了一种新的流动注射 CL 法用于测定雌激素。在最佳实验条件下，得到了三种线性关系。该方法简单、灵敏，已成功用于测定自来水中的雌激素。
