Anhui Key Laboratory of Chemo-Biosensing, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, People's Republic of China.
Luminescence. 2012 Nov-Dec;27(6):466-72. doi: 10.1002/bio.1376. Epub 2012 Jan 4.
A highly sensitive flow-injection chemiluminescence (FIA-CL) method based on the CdTe nanocrystals and potassium permanganate chemiluminescence system was developed for the determination of L-ascorbic acid. It was found that sodium hexametaphosphate (SP), as an enhancer, could increase the chemiluminescence (CL) emission from the redox reaction of CdTe quantum dots with potassium permanganate in near-neutral pH conditions. L-ascorbic acid is suggested as a sensitive enhancer for use in the above energy-transfer excitation process. Under optimal conditions, the calibration graph of emission intensity against logarithmic l-ascorbic acid concentration was linear in the range 1.0 × 10(-9)-5.0 × 10(-6) mol/L, with a correlation coefficient of 0.9969 and relative standard deviation (RSD) of 2.3% (n = 7) at 5.0 × 10(-7) mol/L. The method was successfully used to determine L-ascorbic acid in vitamin C tablets. The possible mechanism of the chemiluminescence in the system is also discussed.
一种基于 CdTe 纳米晶体和高锰酸钾化学发光体系的高灵敏度流动注射化学发光（FIA-CL）方法被开发用于测定 L-抗坏血酸。研究发现，六偏磷酸钠（SP）作为一种增强剂，可以在近中性 pH 条件下增加 CdTe 量子点与高锰酸钾之间的氧化还原反应的化学发光（CL）发射。L-抗坏血酸被建议作为一种用于上述能量转移激发过程的灵敏增强剂。在最佳条件下，发射强度与对数 L-抗坏血酸浓度的校准曲线在 1.0×10(-9)-5.0×10(-6)mol/L 范围内呈线性，相关系数为 0.9969，相对标准偏差（RSD）为 5.0×10(-7)mol/L 时为 2.3%（n=7）。该方法成功地用于测定维生素 C 片剂中的 L-抗坏血酸。还讨论了该体系中化学发光的可能机制。
