National Institute for Cellular Biotechnology, Dublin City University, Dublin 9, Ireland.
Biotechnol Bioeng. 2012 Jun;109(6):1386-94. doi: 10.1002/bit.24476. Epub 2012 Mar 2.
Recently released sequence information on Chinese hamster ovary (CHO) cells promises to not only facilitate our understanding of these industrially important cell factories through direct analysis of the sequence, but also to enhance existing methodologies and allow new tools to be developed. In this article we demonstrate the utilization of CHO specific sequence information to improve mass spectrometry (MS) based proteomic identification. The use of various CHO specific databases enabled the identification of 282 additional proteins, thus increasing the total number of identified proteins by 40-50%, depending on the sample source and methods used. In addition, a considerable portion of those proteins that were identified previously based on inter-species sequence homology were now identified by a larger number of peptides matched, thus increasing the confidence of identification. The new sequence information offers improved interpretation of proteomic analyses and will, in the years to come, prove vital to unraveling the CHO proteome.
最近发布的中国仓鼠卵巢 (CHO) 细胞序列信息不仅有望通过直接分析序列来促进我们对这些工业上重要的细胞工厂的理解，而且还可以增强现有的方法，并开发新的工具。在本文中，我们展示了 CHO 特异性序列信息在提高基于质谱 (MS) 的蛋白质组学鉴定中的利用。使用各种 CHO 特异性数据库可以鉴定 282 种额外的蛋白质，从而使鉴定的蛋白质总数增加了 40-50%，具体取决于样品来源和使用的方法。此外，现在基于种间序列同源性鉴定的大量蛋白质现在可以通过更多匹配的肽来鉴定，从而提高了鉴定的可信度。新的序列信息为蛋白质组学分析提供了更好的解释，并且在未来几年将证明对揭示 CHO 蛋白质组至关重要。
