Department of Pharmacy, University of Patras, 26500 Patras, Greece.
J Mater Sci Mater Med. 2012 May;23(5):1139-48. doi: 10.1007/s10856-012-4593-7. Epub 2012 Mar 3.
In several bone deproteination protocols the chemical agent used for protein cleavage is hydrazine. The effect of hydrazine deproteination method on the crystal size and crystallinity of the bone mineral was studied. Bovine bones were subjected to this protocol and the crystal size and crystallinity of the remaining bone mineral were determined by X-ray Diffraction (XRD), by measuring the width at the half of the maximum intensity of the (002) reflection. It was found that hydrazine deproteination induces noteworthy increase of crystal size and crystallinity. The effect was enhanced by increasing hydrazine temperature from 25 to 55°C. Furthermore, infrared spectroscopy revealed that hydrazine facilitates the removal of carbonate and acid phosphate ions from bone mineral. It is proposed that the mechanism of modification of crystal size and crystallinity lies on the removal of these ions thus, resulting in crystal re-organization.
在几种脱蛋白方案中，用于蛋白质裂解的化学试剂是联氨。本研究旨在探讨联氨脱蛋白方法对骨矿物质晶体大小和结晶度的影响。将牛骨置于该方案中，通过测量（002）反射的最大强度半高处的宽度，利用 X 射线衍射（XRD）来确定剩余骨矿物质的晶体大小和结晶度。结果发现，联氨脱蛋白会显著增加晶体大小和结晶度。通过将联氨温度从 25°C 提高到 55°C，这种效果会增强。此外，红外光谱表明，联氨有助于从骨矿物质中去除碳酸盐和酸性磷酸根离子。据推测，晶体大小和结晶度改变的机制在于这些离子的去除，从而导致晶体重新排列。
