Department of Immunology, Institute of Microbiology, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria.
Phytother Res. 2012 Nov;26(11):1681-7. doi: 10.1002/ptr.4641. Epub 2012 Mar 3.
Verbascum xanthophoeniceum Griseb. is an endemic plant of the Balkan region, a representative of the genus Verbascum used in traditional medicine for respiratory and inflammatory disorders. The objective of this study was to evaluate in vivo and in vitro the antiinflammatory action of crude extract, different fractions and pure compounds obtained from V. xanthophoeniceum Griseb. Bioactive metabolites were isolated by the use of low-pressure chromatographic separation. Crude methanol extract (CME) was applied in a model of paw oedema and different fractions and substances were tested in vitro for their effect on NO and cytokine production by peritoneal macrophages, and on the COX-1 and COX-2 expression. The CME exerted inhibition on cobra venom factor (CVF)-induced oedema in mice, in correlation with reduced alternative pathway (AP) complement activity. A highly suppressive effect was expressed by nigroside VI on IL-6 and NO production and by forsythoside B on TNF-α production. Leucosceptoside B lowered NO release and COX-1 expression in macrophages. Verbascum xanthophoeniceum could serve as a promising source of active compounds with antiinflammatory action, particularly in complement-mediated disorders.
黄花伏毛苣苔（Verbascum xanthophoeniceum Griseb.）是巴尔干地区特有的植物，是伏毛苣苔属植物在传统医学中用于治疗呼吸和炎症性疾病的代表。本研究的目的是评估黄花伏毛苣苔粗提取物、不同馏分和纯化合物的体内和体外抗炎作用。生物活性代谢物是通过低压色谱分离获得的。采用甲醇粗提取物（CME）进行爪肿胀模型实验，测试不同馏分和物质对腹腔巨噬细胞中 NO 和细胞因子产生、COX-1 和 COX-2 表达的影响。CME 对眼镜蛇毒因子（CVF）诱导的小鼠水肿具有抑制作用，与替代途径（AP）补体活性降低有关。黑糖苷 VI 对 IL-6 和 NO 产生以及连翘酯苷 B 对 TNF-α 产生具有高度抑制作用。白毛莨苷 B 降低了巨噬细胞中 NO 的释放和 COX-1 的表达。黄花伏毛苣苔可能是具有抗炎作用的活性化合物的有前途的来源，特别是在补体介导的疾病中。
