Y染色体单核苷酸多态性在法医遗传学中的应用

[Application of Y-SNPs in forensic genetics].

作者信息

Abreu-Głowacka Monica, Koralewska-Kordel Małgorzata, Michalak Eliza, Zaba Czesław, Przybylski Zygmunt

机构信息

Z Katedry i Zakładu Medycyny Sadowej Uniwersytetu Medycznego im. Karola Marcinkowskiego w Poznaniu.

出版信息

Arch Med Sadowej Kryminol. 2011 Apr-Jun;61(2):161-9.

PMID:22390130

Abstract

The study presents the results obtained by LightSNiP method with the use of 2 SNPs located on Y chromosome. The purpose of this study was examination of the mixture of the genetic material in the form of male and female blood in different dilutions. The size of available STR-PCR markers is between 100 and 500 bp, which can limit the possibility of DNA mixture analysis, where the amount of male DNA is relatively low or the genetic material is markedly degraded. Selection of appropriate SNPs placed on Y chromosome can provide information that will facilitate interpretation of the female and male DNA mixture. The test was performed on a Light Cycler 2.0, Roche Diagnostic.

摘要

该研究展示了使用位于Y染色体上的2个单核苷酸多态性（SNP）通过LightSNiP方法获得的结果。本研究的目的是检测不同稀释度下男性和女性血液形式的遗传物质混合物。可用的短串联重复序列-聚合酶链反应（STR-PCR）标记大小在100至500碱基对之间，这可能会限制DNA混合物分析的可能性，即在男性DNA量相对较低或遗传物质明显降解的情况下。选择位于Y染色体上的合适SNP可以提供有助于解释女性和男性DNA混合物的信息。该测试在罗氏诊断公司的Light Cycler 2.0上进行。